Rapid isolation of potent SARS-CoV-2 neutralizing antibodies and protection in a small animal model.

BioRxiv : the Preprint Server for Biology
Thomas F RogersDennis R Burton

Abstract

The development of countermeasures to prevent and treat COVID-19 is a global health priority. In under 7 weeks, we enrolled a cohort of SARS-CoV-2-recovered participants, developed neutralization assays to interrogate serum and monoclonal antibody responses, adapted our high throughput antibody isolation, production and characterization pipeline to rapidly screen over 1000 antigen-specific antibodies, and established an animal model to test protection. We report multiple highly potent neutralizing antibodies (nAbs) and show that passive transfer of a nAb provides protection against high-dose SARS-CoV-2 challenge in Syrian hamsters. The study suggests a role for nAbs in prophylaxis, and potentially therapy, of COVID-19. The nAbs define protective epitopes to guide vaccine design.

Citations

Jun 14, 2020·Nature Reviews. Immunology·Jonathan Abraham
Jul 1, 2020·Turkish Journal of Biology = Türk Biyoloji Dergisi·Murat TopuzoĞullariSerap Derman
Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Oct 8, 2020·Critical Care : the Official Journal of the Critical Care Forum·Salleh N EhaidebAbderrezak Bouchama
Sep 10, 2020·Journal of Virology·Rebecca L BrocatoJay W Hooper
Aug 4, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Sebastian HörberAndreas Peter
Oct 17, 2020·Annual Review of Biophysics·Jiahui ChenGuo-Wei Wei
Jun 11, 2021·Frontiers in Molecular Biosciences·Sonia Beeckmans, Edilbert Van Driessche
Sep 2, 2021·Molecular Biology·A N VzorovA L Gintsburg

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PCR
bio-layer interferometry
biosensors
surface plasmon resonance

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.