Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis.

Scientific Reports
Rong LeiNing Jiang

Abstract

Equine piroplasmosis (EP) is a severe disease of horses caused by the tick-borne protozoa Theileria equi (T. equi) and Babesia caballi (B. caballi). Infectious carriers are not always symptomatic, meaning there is a risk to non-enzootic areas. Regulatory tests for EP include sero-epidemiological methods for equine babesiosis, but these lack specificity due to cross-reactivity with other Babesia species. In this study, we present a real-time quantitative recombinase polymerase amplification (qRPA) method for fast simultaneous detection of both T. equi and B. caballi. In this method, primers and probes targeting the 18S rRNA gene of both T. equi and B. caballi, the ema-1 gene of T. equi and the bc48 gene of B. caballi were designed and evaluated. The sensitivity of qRPA was evaluated using the pUC57 plasmid DNA containing the target gene. For the pUC57-bc48 gene DNA, the R2 value was 0.983 for the concentration range 0.2 ng (4.1 × 107 DNA copies) to 2.0 fg (4.1 × 101 DNA copies). For the pUC57-ema gene DNA, the R2 value was 0.993 for the concentration range 0.2 ng (5.26 × 107 DNA copies) to 2.0 fg (5.26 × 102 DNA copies). For the pUC57-Bc18S gene DNA the R2 value was 0.976 for the concentration range 2.0 ng (4.21 × 108 DNA copies...Continue Reading

References

Dec 11, 1992·Journal of Clinical Microbiology·D P KnowlesL E Perryman
Jan 1, 1992·The British Veterinary Journal·D T de Waal
Jan 1, 1991·The Veterinary Clinics of North America. Small Animal Practice·J Taboada, S R Merchant
Sep 1, 1996·Revue Scientifique Et Technique·K T Friedhoff, C Soulé
Jul 11, 1998·Parasitology Research·H Mehlhorn, E Schein
Oct 6, 2001·Veterinary Parasitology·T B NicolaiewskyL S Ozaki
Jun 9, 2005·Parasitology·F Jongejan, G Uilenberg
Mar 4, 2006·Veterinary Parasitology·Gerrit Uilenberg
Jul 3, 2010·The American Journal of Tropical Medicine and Hygiene·Birgit PöschlPanagiotis Karanis
Sep 17, 2013·Journal of Veterinary Internal Medicine·L N WiseD P Knowles
May 31, 2014·Analytical Chemistry·Zachary Austin CrannellRebecca Richards-Kortum
Dec 17, 2014·The American Journal of Tropical Medicine and Hygiene·Zachary Austin CrannellRebecca Richards-Kortum
Dec 17, 2015·Analytical Chemistry·Zachary Austin CrannellRebecca Richards-Kortum
May 11, 2016·Clinical Chemistry·Rana K DaherMichel G Bergeron
Nov 9, 2016·The American Journal of Tropical Medicine and Hygiene·Miguel M CabadaA Clinton White
Oct 5, 2017·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Maria do Socorro Costa de Oliveira BragaRosangela Zacarias Machado
Dec 21, 2017·The American Journal of Tropical Medicine and Hygiene·Meng-Yee LaiYee-Ling Lau
Jan 2, 2018·Acta Tropica·Maria Isabel Botelho VieiraRosangela Zacarias Machado
Feb 17, 2018·Acta Tropica·A Castellanos-GonzalezB Travi
Mar 4, 2018·Parasites & Vectors·Vladislav A LobanovAlvin A Gajadhar
Nov 26, 2018·Ticks and Tick-borne Diseases·M G Montes CortésM Á Habela Martínez-Estéllez

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
DNA assay
Assay

Software Mentioned

Oligoanalyzer

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Babesiosis (ASM)

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.