Rapid killing of murine lymph node T blasts by intestinal intraepithelial lymphocytes in vitro

European Journal of Immunology
Y KawamotoAkio Miyama

Abstract

We examined the effect of murine intestinal intraepithelial lymphocytes (IEL) on the proliferation of murine lymph node T cells (LN-T) in vitro. An IEL fraction prevented the proliferation of LN-T stimulated with antigen and X-irradiated spleen cells, or with anti-CD3 monoclonal antibodies (mAb). Concanavalin A-activated LN-T were less sensitive. Such an inhibitory activity was recovered from a CD8-depleted population by panning of bulk IEL using anti-CD8 alpha mAb. This population of BALB/c IEL showed less granzyme A activity, and its surface markers were positive for CD8 (4%), CD3 (80-90%), CD4 (2-6%), alpha-beta TcR (45-70%), and gamma-delta TcR (4-9%). Asialo-GM1 and Thy1.2 were variably expressed, but interleukin-2 (IL-2) receptor-alpha and Fc gamma receptor were not. By contrast, no cytotoxicity against YAC-1 was detected in a CD8-depleted IEL population by a 6-h 51Cr-release assay. Although IEL from severe-combined immunodeficient mice lacking CD4, CD8 and TcR, but expressing IL-2 receptor, showed cytotoxicity against YAC-1, their inhibitory activity against LN-T was almost the same as that by IEL from BALB/c mice. When LN-T blasts (greater than 75% CD4+) activated with anti-CD3 were treated with CD8-depleted IEL, intact...Continue Reading

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Citations

Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Phillip D HollerDiane Mathis
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·Toshiyasu ShimizuTakao Tsuji
Jan 3, 2001·Blood·T A Fehniger, M A Caligiuri
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sheng F CaiTimothy J Ley

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