Rapid kinetics and inward rectification of miniature EPSCs in layer I neurons of rat neocortex

Journal of Neurophysiology
F M Zhou, J J Hablitz

Abstract

With the use of the whole cell patch-clamp technique combined with visualization of neurons in brain slices, we studied the properties of miniature excitatory postsynaptic currents (mEPSCs) in rat neocortical layer I neurons. At holding potentials (-50 to -70 mV) near the resting membrane potential (RMP), mEPSCs had amplitudes of 5-100 pA and were mediated mostly by alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionate (AMPA) receptors. Amplitude histograms were skewed toward large events. An N-methyl-D-aspartate (NMDA) component was revealed by depolarization to -30 mV or by the use of a Mg2+-free bathing solution. At RMP, averaged AMPA mEPSCs had a 10-90% rise time of approximately 0.3 ms (uncorrected for instrument filtering). The decay of averaged mEPSCs was best fit by double-exponential functions in most cases. The fast, dominating component had a decay time constant of approximately 1.2 ms and comprised approximately 80% of the total amplitude. A small slow component had a decay time constant of approximately 4 ms. Positive correlations were found between rise and decay times of both individual and averaged mEPSCs, indicative of dendritic filtering. Some large-amplitude mEPSCs and spontaneous EPSCs (recorded in the absen...Continue Reading

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Aug 1, 1996·Journal of Neurophysiology·F M Zhou, J J Hablitz

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Citations

Mar 23, 1999·Journal of Neurophysiology·F M Zhou, J J Hablitz
Aug 30, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D V V SantosM Da Silva Filho
Dec 22, 1999·Journal of Neurophysiology·F M Zhou, J J Hablitz
Nov 14, 1997·Journal of Neurophysiology·F M Zhou, J J Hablitz
Dec 4, 2001·Journal of Neurophysiology·M EnnisM T Shipley
Dec 27, 2018·The European Journal of Neuroscience·Ying-Wan Lam, S Murray Sherman
Mar 30, 2013·Neuropharmacology·Alexandre W Moreau, Dimitri M Kullmann

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