Rapid Legionella pneumophila determination based on a disposable core-shell Fe₃O₄@poly(dopamine) magnetic nanoparticles immunoplatform

Analytica Chimica Acta
Miriam MartínJosé Luis González-Mora

Abstract

A novel amperometric magnetoimmunoassay, based on the use of core-shell magnetic nanoparticles and screen-printed carbon electrodes, was developed for the selective determination of Legionella pneumophila SG1. A specific capture antibody (Ab) was linked to the poly(dopamine)-modified magnetic nanoparticles (MNPs@pDA-Ab) and incubated with bacteria. The captured bacteria were sandwiched using the antibody labeled with horseradish peroxidase (Ab-HRP), and the resulting MNPs@pDA-Ab-Legionella neumophila-Ab-HRP were captured by a magnetic field on the electrode surface. The amperometric response measured at -0.15 V vs. Ag pseudo-reference electrode of the SPCE after the addition of H2O2 in the presence of hydroquinone (HQ) was used as transduction signal. The achieved limit of detection, without pre-concentration or pre-enrichment steps, was 10(4) Colony Forming Units (CFUs) mL(-1). The method showed a good selectivity and the MNPs@pDA-Ab exhibited a good stability during 30 days. The possibility of detecting L. pneumophila at 10 CFU mL(-1) level in less than 3 h, after performing a membrane-based preconcentration step, was also demonstrated.

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Citations

Dec 17, 2016·Infectious Disease Clinics of North America·W Michael DunneAlex van Belkum
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Dec 16, 2017·Sensors·Celine I L JustinoTeresa A P Rocha-Santos
Nov 24, 2018·Journal of Nanobiotechnology·Marjorie R Willner, Peter J Vikesland
Sep 21, 2019·Advanced Materials·Lucy GloagJ Justin Gooding
May 5, 2020·Biosensors & Bioelectronics·Ellen Cesewski, Blake N Johnson
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob
Sep 17, 2018·International Journal of Biological Macromolecules·Ahmad MobedJavid Sadeghi

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