Rapid liquid chromatography/tandem mass spectrometer (LCMS) method for clozapine and its metabolite N-desmethyl clozapine (norclozapine) in human serum.

Journal of Clinical Laboratory Analysis
L V RaoG M Vallaro

Abstract

Clozapine is indicated for the treatment of schizophrenia and related psychotic disorders. Several methods have been developed for monitoring Clozapine levels; however, they possess limited specificity and are often laborious. This study describes a simple liquid chromatography/tandem mass spectrometer (LCMS) method in human serum. The ion transitions monitored were m/z 327, 270, 296 for Clozapine, m/z 313, 192, 227 for Norclozapine and m/z 328, 271 for Loxapine. The assay is linear (25-1000 ng/ml) and showed a good correlation (r=0.98) within the analytical range of 79-1210 ng/ml in human serum. This assay is highly specific and sensitive for the simultaneous measurements of Clozapine and Norclozapine. The simplification of this assay makes it ideal for high throughput analyses of the patient samples in a routine clinical laboratory staffed with general medical technologists.

References

Dec 30, 1988·Journal of Chromatography·K Richter
Jan 1, 1994·The Journal of Neuropsychiatry and Clinical Neurosciences·J WelchD Redmond
Jan 1, 1995·The Journal of Neuropsychiatry and Clinical Neurosciences·E D RisbyR L Lewine
Aug 1, 1997·Therapeutic Drug Monitoring·H WeigmannC Hiemke
Aug 29, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·K K Akerman
May 24, 2001·Progress in Neuro-psychopharmacology & Biological Psychiatry·L PalegoG B Cassano
Jun 8, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·A LlerenaA de la Rubia
Aug 10, 2001·Drug Metabolism Reviews·Z H Israili, P G Dayton
May 4, 2002·Canadian Journal of Psychiatry. Revue Canadienne De Psychiatrie·L Kola OyewumiDavid L Streiner
Mar 11, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·H A G NiederländerG J de Jong
Oct 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laura MercoliniMaria Augusta Raggi
Dec 8, 2006·The American Journal of Psychiatry·Daniel PolskyHenry A Glick

❮ Previous
Next ❯

Citations

May 11, 2016·Clinical Biochemistry·Maria Shipkova, Dobrin Svinarov
Jun 16, 2010·Journal of Separation Science·Ulrike HolzgrabeStefan Almeling
Jun 14, 2017·Multiple Sclerosis Journal - Experimental, Translational and Clinical·Laura K GreenAnne Camille La Flamme

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here