PMID: 9466742Feb 18, 1998

Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay

Journal of Clinical Microbiology
S G FranzblauRobert H Gilman

Abstract

A colorimetric, microplate-based Alamar Blue assay (MABA) method was used to determine the MICs of isoniazid (INH), rifampin, streptomycin (SM), and ethambutol (EMB) for 34 Peruvian Mycobacterium tuberculosis isolates (including both pansensitive and multidrug-resistant strains) and the H37Rv strain by using bacterial suspensions prepared directly from solid media. Results for all isolates were available within 8 days. Discordant results were observed on initial tests for 3 of 16 INH-susceptible isolates, 5 of 31 EMB-susceptible isolates, and 2 of 4 SM-resistant isolates (by the BACTEC 460 system). The overall agreements between the MICs obtained by MABA and the results obtained with the BACTEC 460 system were 87.9% for initial results and 93.6% after retesting 12 of 17 samples with discrepant results. Interpretation of MABA endpoints improved with technical experience. The MABA is a simple, rapid, low-cost, appropriate technology which does not require expensive instrumentation and which makes use of a nontoxic, temperature-stable reagent.

Citations

Aug 26, 2004·Antimicrobial Agents and Chemotherapy·Rosilene Fressatti CardosoMário Hiroyuki Hirata
Jun 25, 2008·Archiv der Pharmazie·Amit TrivediViresh Shah
Sep 8, 2009·The Journal of Antimicrobial Chemotherapy·Kathleen EnglandRichard A Slayden
Nov 3, 2010·Chemical Biology & Drug Design·Sandip B Bharate, Charles M Thompson
Jan 1, 2009·Pharmaceutical Biology·Bethany G ElkingtonD Doel Soejarto
Jun 16, 2011·Chemistry & Biodiversity·María F PlanoRaquel M Cravero
Mar 27, 2012·The Journal of Infectious Diseases·Geoffrey D CoxonTimothy D McHugh
Nov 15, 2012·Chemical & Pharmaceutical Bulletin·Pichit SudtaSunit Suksamrarn
Mar 19, 2014·Chemical Biology & Drug Design·Variam Ullas JeankumarDharmarajan Sriram
Feb 1, 2010·Helvetica Chimica Acta·Qing LiuYing-Jun Zhang
Jan 31, 2014·Pathogens and Disease·David F AckartRandall J Basaraba
May 11, 2015·The Journal of Antimicrobial Chemotherapy·Ningyu ZhuShuyi Si
Dec 24, 2014·Antimicrobial Agents and Chemotherapy·Trudy H GrossmanChristopher P Locher
Sep 16, 2015·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Begüm Evranos-AksözSelda Özgen Özgacar
Jan 26, 2016·ChemMedChem·Variam Ullas JeankumarDharmarajan Sriram
Mar 15, 2016·International Journal of Mycobacteriology·Manoj ChandranDharmarajan Sriram
Sep 22, 2016·International Journal of Mycobacteriology·Joseph Mwanzia NgutaPhyllis G A Addo
Oct 30, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Claudia CortesiaHoward Takiff
Feb 10, 2017·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Patel NavinGildardo Rivera
Nov 23, 2019·Current Topics in Medicinal Chemistry·Deyani Nocedo-MenaMaría Del Rayo Camacho-Corona
Jan 10, 2020·Future Medicinal Chemistry·Vasilichia AntociIonel I Mangalagiu
Jun 18, 2002·Infection and Immunity·C A Brissette, S A Lukehart
Apr 13, 2005·The Journal of Antimicrobial Chemotherapy·Remedios GunaRafael Borrás
May 7, 2008·Antimicrobial Agents and Chemotherapy·Olga DanilchankaMichael Niederweis
Jan 11, 2007·Antimicrobial Agents and Chemotherapy·Sang-Hyun ChoS G Franzblau
Jul 16, 2008·Antimicrobial Agents and Chemotherapy·Esther VicenteRobert C Goldman
Jul 25, 2009·Medicinal Research Reviews·Arvind S NegiSuman P S Khanuja
Dec 21, 2006·Antimicrobial Agents and Chemotherapy·Poonpilas HongmaneePrasit Palittapongarnpim
Feb 13, 2007·Memórias do Instituto Oswaldo Cruz·Rosilene Fressatti CardosoMário Hiroyuki Hirata
Jun 19, 2008·Antimicrobial Agents and Chemotherapy·Olga DanilchankaMichael Niederweis
May 19, 2007·Journal of Medical Microbiology·Jorge Castro-GarzaLucio Vera-Cabrera
Mar 19, 2011·Chemical Biology & Drug Design·Silvia H CardosoMarcus V N de Souza
Mar 12, 2011·The Journal of Antimicrobial Chemotherapy·Paula Aline Zanetti CampanerutRosilene Fressatti Cardoso
Nov 15, 2011·Chemical Biology & Drug Design·Cristiane F Da CostaMarcus V N de Souza
Jan 26, 2010·Archiv der Pharmazie·João P S FernandesCarlos A Brandt
May 14, 2014·FEMS Microbiology Letters·Jie LuZhuo Zhao
Mar 20, 2013·BioMed Research International·Daiane MartinsCecilia Veronica Nunez
Oct 5, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Reshma AlokamDharmarajan Sriram
Dec 23, 2014·Chemical Biology & Drug Design·Celso O RezendeMauro V Almeida
Jul 6, 2013·Asian Pacific Journal of Tropical Medicine·Gomathi SivaramakrishnanVanaja Kumar
Jun 21, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Joana Darc Souza ChavesMauro Vieira de Almeida
Feb 24, 2016·Antimicrobial Agents and Chemotherapy·C Seth PearsonGeorges Belfort
Sep 7, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Gisele S S FirminoJosane A Lessa
Oct 6, 2015·Nature Reviews. Drug Discovery·Kei KatsunoB T Slingsby
Dec 29, 2016·Archives of Pharmacal Research·Hajjaj H M Abdu-AllahDharmarajan Sriram
Oct 27, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Anda-Mihaela OlaruIonel I Mangalagiu
Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weronika AmbrożkiewiczJan Zitko
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·Rosanna MaccariMaria Gabriella Vigorita
Mar 29, 2005·Antimicrobial Agents and Chemotherapy·Kanakeshwari FalzariS G Franzblau
Jun 20, 2000·Antimicrobial Agents and Chemotherapy·C S Lunde, I Kubo
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·Guoyi MaIkhlas A Khan
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jun-Rong WeiEric J Rubin
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Juan-Carlos PalominoFrançoise Portaels
Oct 20, 2009·Applied and Environmental Microbiology·James CarrollJim O'Mahony
Mar 15, 2008·Phytotherapy Research : PTR·Delia Elva Cruz-VegaJorge Castro-Garza
Jul 10, 2012·Current Microbiology·Fernanda da C SantosMaria C B V de Souza
May 19, 2010·Expert Review of Respiratory Medicine·Elvira RichterDoris Hillemann
Oct 31, 2006·Memórias do Instituto Oswaldo Cruz·Luciano MengattoMaría Susana Imaz
Jan 29, 2008·Chemical Biology & Drug Design·Márcia da SilvaKátia Cirlene Alves Botelho
Sep 17, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·Tanu ChamoliMelissa Jacob
Mar 22, 2014·Natural Product Research·Muthyala Murali Krishna KumarDesaraju Venkata Rao
Dec 5, 2012·Antimicrobial Agents and Chemotherapy·Y PangYanlin Zhao
Nov 26, 2013·BMC Complementary and Alternative Medicine·Ramón Enrique Robles-ZepedaAdriana Garibay-Escobar
Oct 27, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Z ZhangY Zhao
Oct 14, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Areej AbuhammadEdith Sim
May 3, 2016·Archiv der Pharmazie·Sivakumar Kullampalayam KrishnasamySenthilkumar Palaniappan
Jun 4, 2020·Marine Drugs·Sara García-DavisEzequiel Viveros-Valdez
Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martin JuhásJan Zitko
Sep 13, 2006·Antimicrobial Agents and Chemotherapy·Jeanette W P TeoKakoli Mukherjee
Feb 3, 2000·Memórias do Instituto Oswaldo Cruz·Clarice Q F LeiteM A Telles
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rachel J DuttonDana Boyd
Oct 13, 2006·The New England Journal of Medicine·David A J MooreJon S Friedland
May 27, 2008·The Journal of Antimicrobial Chemotherapy·Raquel VillarRobert C Goldman
Oct 2, 2012·Medicinal Chemistry Research : an International Journal for Rapid Communications on Design and Mechanisms of Action of Biologically Active Agents·Jiangtao GaoStephen J Cutler
Nov 19, 2008·Antimicrobial Agents and Chemotherapy·Agustina I de la IglesiaHéctor R Morbidoni
Sep 16, 2011·The Journal of Antimicrobial Chemotherapy·Rashmi TandonMridula Bose
Apr 24, 2004·Antimicrobial Agents and Chemotherapy·Clinton K Murray, Duane R Hospenthal
Sep 29, 2005·Antimicrobial Agents and Chemotherapy·Lucio Vera-CabreraCarmen Molina-Torres
Dec 19, 2008·Evidence-based Complementary and Alternative Medicine : ECAM·C T HiguchiC Q F Leite
May 12, 2006·Archiv der Pharmazie·Marcia SilvaElizabeth I Ferreira
May 14, 2016·PloS One·Heather L TorreyKim Lewis
Jul 24, 2012·Marine Drugs·Sridevi Ankisetty, Marc Slattery
May 11, 2013·The Journal of Biological Chemistry·Christofer BjörkelidT Alwyn Jones
Jul 8, 2015·Antimicrobial Agents and Chemotherapy·Ragini SinghSantanu Datta
Feb 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ondrej JandourekMartin Dolezal
Sep 19, 2019·Molecular Biology and Evolution·Rhastin A D CastroSebastien Gagneux
Jun 24, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Muhib AhmedAndré Luis Souza Dos Santos
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irina ZarafuCristiana Radulescu
Jul 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tarek S IbrahimAmany M M Al-Mahmoudy
Nov 26, 2003·Antimicrobial Agents and Chemotherapy·Glenn P MorlockRobert C Cooksey
Jan 1, 2012·Pharmaceuticals·Neeraj ShakyaRakesh Kumar
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yuan LinShuyi Si
Jun 6, 2009·Molecular Microbiology·Partha MukherjeeJoyoti Basu
Nov 12, 2013·Molecular Microbiology·Ruchi Pandey, G Marcela Rodriguez
Dec 24, 2010·Journal of Clinical Microbiology·Xia YuHairong Huang
Mar 20, 2013·Antimicrobial Agents and Chemotherapy·Suresh SolapureV Balasubramanian
Oct 18, 2015·Scientia pharmaceutica·Emerson T da SilvaSolange M S V Wardell
Jun 11, 2015·Chemical Biology & Drug Design·Elena MatyuginaAnastasia L Khandazhinskaya
Feb 22, 2001·Antimicrobial Agents and Chemotherapy·P E SilvaJ A Aínsa
Mar 9, 2011·Chemical Communications : Chem Comm·Nicholas P WestRichard J Payne
Apr 1, 2014·Marine Drugs·Cristina I Canché ChayRoberto Martínez
Sep 4, 2014·Asian Pacific Journal of Tropical Biomedicine·Abiodun FalodunMark Hamann
Nov 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marta Kucerova-ChlupacovaVeronika Opletalova

Related Concepts

Alamar Blue
Antibiotics, Antitubercular
Antitubercular Agents
Bacteriological Techniques
Antibiotic Resistance, Microbial
Organic Pigments
Etambutol Llorente
Tubazide
Fungus Drug Sensitivity Tests
Mycobacterium tuberculosis H37Rv

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