PMID: 9187387Apr 25, 1997Paper

Rapid method to determine sphinganine/sphingosine in human and animal urine as a biomarker for fumonisin exposure

Journal of Chromatography. B, Biomedical Sciences and Applications
M SolfrizzoA Visconti

Abstract

The widespread occurrence of fumonisins in maize and maize-based foods and feeds demands the development of rapid and reliable methods for the analysis of suitable biomarkers in biological fluids in order to assess human and animal exposure to these important mycotoxins. The increase in the ratio of free sphinganine/sphingosine (SA/SO) in urine has been recently proposed as a biomarker to evaluate exposure to fumonisins. The presently available method for the determination of SA and SO in biological samples is labor intensive, time consuming and insufficiently accurate. A new method has been proposed for the determination of SA and SO in human and animal urine which is more precise and accurate, and drastically reduces the number of steps during extraction and clean-up. The method is essentially based on the use of silica minicolumn clean-up of the chloroform extract from alkalinized urine. The final extract is derivatized with o-phthaldialdehyde reagent and SO and SA are determined by reversed-phase HPLC with fluorimetric detector. Urine samples spiked with SO, SA standards at concentrations ranging from 1.5 to 15 ng/ml have given mean recoveries higher than 80% and precision (coefficient of variation) lower than 10%. Detectio...Continue Reading

References

Sep 5, 1990·Journal of the National Cancer Institute·S FranceschiC La Vecchia
Jan 1, 1995·Natural Toxins·W F Marasas
Jan 1, 1995·Natural Toxins·G S ShephardM E Savard
Jul 1, 1994·Food Additives and Contaminants·M B Doko, A Visconti
May 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·G S ShephardD J van Schalkwyk
Jan 1, 1996·Advances in Experimental Medicine and Biology·A Visconti

❮ Previous
Next ❯

Citations

Apr 21, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M SolfrizzoA Visconti
Mar 15, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R KroesA Visconti
Feb 2, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L van der WesthuizenG S Shephard
Oct 18, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·W SeefelderH-U Humpf
Aug 25, 2001·The British Journal of Nutrition·H CrewsG Williamson
Sep 13, 2001·Food Additives and Contaminants·L GarrenM Castegnaro
Jul 16, 2005·Food Additives and Contaminants·G AvantaggiatoA Visconti
Oct 25, 2006·Journal of Toxicology and Environmental Health. Part a·Dong-Hyun KimSeikwan Oh
May 22, 2001·Environmental Health Perspectives·W F Marasas
Jan 12, 2010·Arhiv za higijenu rada i toksikologiju·Ana-Marija DomijanRadovan Fuchs
May 30, 2009·Toxicon : Official Journal of the International Society on Toxinology·Liliana J G SilvaAngelina Pena
Dec 15, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M G TheumerH R Rubinstein
Aug 11, 2016·Journal of Agricultural and Food Chemistry·Lucia GambacortaMichele Solfrizzo
Oct 2, 2007·Toxicology and Applied Pharmacology·Qingsong CaiJia-Sheng Wang
Jul 5, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Shih-Hsuan HsiaoWanda M Haschek
Aug 11, 2017·Nutritional Neuroscience·Barbara De SantisMaria Elisabetta Raggi
Mar 2, 2019·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Ruth Nabwire WangiaJia-Sheng Wang
Oct 5, 2001·The Journal of Nutrition·C C AbnetS M Dawsey
Feb 9, 2021·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Ruth Nabwire Wangia-Dixon, Kizito Nishimwe
Aug 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·G S Shephard, L van der Westhuizen
Jan 19, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M CastegnaroC P Wild

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.