Rapid micro-method for the measurement of phenobarbitone, primidone and phenytoin in blood plasma or serum by gas-liquid chromatography

Journal of Chromatography
D M Rutherford, R J Flanagan

Abstract

A rapid gas-liquid chromatographic method has been developed for the analysis of phenobarbitone, primidone and phenytoin in small (50 microliter) volumes of either blood plasma or serum. Neither solvent transfer nor evaporation are required in the extraction, which takes less than 3 min to complete, and a quantitative analysis may be performed, in duplicate, within 20 min. Sources of interference in the assay are minimal, and prior treatment of the column with gamma-glycidoxypropyltrimethoxysilane facilitates the measurement of as little as 10 ng of underivatized drug "on-column" using a flame-ionisation detector. The method has proved valuable when used for detection and measurement of these three compounds at concentrations of 2 mg/1 or greater.

References

Jan 21, 1977·Journal of Chromatography·R E Hill, A N Latham
May 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·P A ToselandD J Berry

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Citations

Sep 1, 1979·Pharmacology, Biochemistry, and Behavior·C E LauJ L Falk
May 10, 1985·Journal of Chromatography·J T Burke, J P Thénot
May 15, 1987·Journal of Chromatography·J M Streete, D J Berry
Oct 6, 1979·British Medical Journal·M HelliwellR J Flanagan
May 14, 1982·Journal of Chromatography·A Di CorciaR Samperi

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