Rapid Microbiology Screening in Pharmaceutical Workflows

SLAS Technology
C SurretteC M Puleo

Abstract

Recently advances in miniaturization and automation have been utilized to rapidly decrease the time to result for microbiology testing in the clinic. These advances have been made due to the limitations of conventional culture-based microbiology methods, including agar plate and microbroth dilution, which have long turnaround times and require physicians to treat patients empirically with antibiotics before test results are available. Currently, there exist similar limitations in pharmaceutical sterility and bioburden testing, where the long turnaround times associated with standard microbiology testing drive costly inefficiencies in workflows. These include the time lag associated with sterility screening within drug production lines and the warehousing cost and time delays within supply chains during product testing. Herein, we demonstrate a proof-of-concept combination of a rapid microfluidic assay and an efficient cell filtration process that enables a path toward integrating rapid tests directly into pharmaceutical microbiological screening workflows. We demonstrate separation and detection of Escherichia coli directly captured and analyzed from a mammalian (i.e., CHO) cell culture with a 3.0 h incubation. The demonstratio...Continue Reading

References

May 24, 2016·Lab on a Chip·Tomasz S KaminskiPiotr Garstecki
Mar 2, 2017·Nature Reviews. Urology·Michael DavenportJoseph C Liao
Jul 16, 2017·Biotechnology and Bioengineering·Mo JiangRichard D Braatz
Mar 3, 2018·Journal of Pharmaceutical Innovation·Adriana BugnoTerezinha de Jesus Andreoli Pinto

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Methods Mentioned

BETA
PCR
fluorescence microscopy

Software Mentioned

FIJI
ImageJ

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