Rapid microscopy technique for detection of Pneumocystis carinii in fresh clinical specimens

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
G ChichinoM Scaglia

Abstract

A direct method for detection of Pneumocystis carinii was evaluated in 14 patients with impaired immune function (3 seropositive for HIV, 8 with AIDS and 3 with heart transplants) and signs and symptoms suggestive of Pneumocystis carinii pneumonia. Direct examination by phase-contrast and interference-contrast microscopy of fresh clinical specimens obtained by sputum induction, bronchoalveolar lavage or transbronchial lung biopsy was found to be a simple and rapid method for detection of Pneumocystis carinii, the sensitivity of the method being comparable to that of the classical toluidine blue O and Diff-Quik staining methods. These findings suggest that this direct microscopy technique could be considered for routine clinical application in patients with suspected Pneumocystis carinii pneumonia.

Citations

Apr 1, 1977·Pediatric Research·L L PiferM J Murphy
Jan 1, 1978·The Journal of Experimental Medicine·H Masur, T C Jones
Jan 1, 1986·Journal of Clinical Microbiology·J J RuffoloP D Walzer
May 1, 1989·Experimental Parasitology·S L StringerM T Cushion
Aug 1, 1986·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·T ShiotaY Yoshida
Nov 1, 1986·Reviews of Infectious Diseases·J Mills

Related Concepts

Lung
Pneumocystis Infections
Bronchoalveolar Lavage
Sputum, Induced
Rapid Technique
Tolonium chloride
Pneumocystis jiroveci
Sputum
Microscopy
HIV Seropositivity

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