Rapid microtiter plate assay for determination of inulin in human plasma and dialysate

Journal of Pharmaceutical and Biomedical Analysis
Thomas D NolinReginald F Frye

Abstract

A rapid, sensitive, and reproducible microtiter plate assay for the determination of inulin in human plasma, dialysate, and phosphate-buffered saline (PBS) was developed. Plasma or PBS samples (100 microl aliquots) were prepared by the addition of indole-3-acetic acid (150 microl) and HCl (3 ml) and then briefly vortex-mixed. Samples were then incubated in a 60 degrees C water bath for 20 min, cooled in a room temperature water bath for 40 min, then diluted with deionized, distilled water (DDW; 3 ml) and again vortex-mixed. Finally, an aliquot (200 microl) of each sample was transferred to a 96-well microtiter plate and read spectrophotometrically at 490 nm. Dialysate samples were processed in a similar manner, but required an initial enzymatic step in order to remove dextrose and minimize assay interference. Samples (100 microl aliquots) were prepared by the addition of glucose oxidase/catalase solution (100 microl), briefly vortex mixed, and then incubated in a 37 degrees C water bath for 60 min, samples were then reacted with indole-3-acetic acid as before. Calibration curves were linear over the concentration range of 0.5-4 mg/ml or 0.025-0.4 mg/ml for plasma or PBS and dialysate, respectively; correlation coefficients (r(2...Continue Reading

References

Oct 1, 1977·AJR. American Journal of Roentgenology·N R DunnickR A Castellino
Jan 10, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·T I RuoA J Atkinson
Apr 25, 1991·Nucleic Acids Research·G J OlsenC R Woese
Nov 1, 1995·Annals of Clinical Biochemistry·O SugitaN Kaneda
Oct 6, 1995·Journal of Chromatography. B, Biomedical Applications·R Dall'AmicoF Zacchello
Aug 26, 1998·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J K LeypoldtR B Deeter
Jan 23, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·M K ScottW R Clark
Feb 19, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J K LeypoldtF K Port
May 29, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·M C Yang, C C Lin

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Citations

Oct 17, 2002·Analytical Biochemistry·Virginie Laurent, Iris Lindberg
Apr 10, 2012·Analytical Biochemistry·Takahiro RikitaToshinobu Seki
Oct 20, 2018·The Journal of Physiology·Bożena BądzyńskaJanusz Sadowski
Feb 15, 2018·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Olga GawryśElżbieta Kompanowska-Jezierska
Jun 8, 2007·Journal of the American Society of Nephrology : JASN·Enqi LiuJianglin Fan
Feb 5, 2005·Medicine and Science in Sports and Exercise·Geneviève C MajorSimone Lemieux
Mar 10, 2021·Clinical and Experimental Hypertension : CHE·Malwina M Roszkowska-ChojeckaElzbieta Kompanowska-Jezierska

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