Rapid mineralization of electrospun scaffolds for bone tissue engineering

Journal of Biomaterials Science. Polymer Edition
Tea AndricJoseph W Freeman

Abstract

We investigated different techniques to enhance calcium phosphate mineral precipitation onto electrospun poly((L)-lactide) (PLLA) scaffolds when incubated in concentrated simulated body fluid (SBF), 10×SBF. The techniques included the use of vacuum, pre-treatment with 0.1 M NaOH and electrospinning gelatin/PLLA blends as means to increase overall mineral precipitation and distribution throughout the scaffolds. Mineral precipitation was evaluated using environmental scanning electron microscopy, energy dispersive spectroscopy mapping and the determination of the mineral weight percents. In addition we evaluated the effect of the techniques on mechanical properties, cellular attachment and cellular proliferation on scaffolds. Two treatments, pre-treatment with NaOH and incorporation of 10% gelatin into PLLA solution, both in combination with vacuum, resulted in significantly higher degrees of mineralization (16.55 and 15.14%, respectively) and better mineral distribution on surfaces and through the cross-sections after 2 h of exposure to 10×SBF. While both scaffold groups supported cell attachment and proliferation, 10% gelatin/PLLA scaffolds had significantly higher yield stress (1.73 vs 0.56 MPa) and elastic modulus (107 vs 44 ...Continue Reading

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Citations

Oct 28, 2011·Tissue Engineering. Part B, Reviews·Caroline SzpalskiStephen M Warren
Jan 18, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Paola TorricelliAdriana Bigi
Jan 5, 2013·Experimental Eye Research·Amy P Lynch, Mark Ahearne
May 23, 2012·Journal of Biomedical Materials Research. Part a·Tea AndricJoseph W Freeman
Mar 29, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Brittany L TaylorJoseph W Freeman
Jul 8, 2017·Biomaterials Science·Yu WenLu Shibi
Feb 21, 2019·Journal of Biomedical Materials Research. Part a·Wei JingWeihong Zhong
Nov 7, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·James CiprianoJoseph W Freeman
Nov 17, 2020·Regenerative Engineering and Translational Medicine·Brittany L TaylorJoseph Freeman

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