Rapid molecular testing for multi-resistant tuberculosis in Mongolia: A diagnostic accuracy study

International Journal of Mycobacteriology
B BuyankhishigSatoshi Mitarai

Abstract

The aim of this study was to assess the performance of a molecular line probe assay, GenoType® MTBDRplus, for rapid detection of rifampicin and isoniazid resistance in the Mongolian situation. The sensitivity and specificity of GenoType® MTBDRplus to detect rifampicin and isoniazid resistance-associated mutations in culture specimens and directly in smear-positive clinical specimens was examined. 218 MDR-TB subjects aged between 14 and 75years old from eight districts in Ulaanbaatar city (between July 2009 and May 2010) were included in this study .The GenoType Mycobacterium tuberculosis drug resistance first line (MTBDR plus) assay (Hain Life-science, Nehren, Germany) was tested on 109 clinical isolates and directly on 41 sputum specimens for the ability to detect the resistances. Results were compared with conventional culture and drug susceptibility testing on solid medium. The high correlation of the results from GenoType® MTBDRplus and conventional drug susceptibility testing was obtained from this study. The results clearly showed a high performance of GenoType® MTBDRplus with almost 100% accuracy for all the important indicators, such as sensitivity, specificity, positive and negative predictive values and detection of r...Continue Reading

References

Jan 1, 2003·Clinical Chemistry·Patrick M BossuytUNKNOWN Standards for Reporting of Diagnostic Accuracy
Jan 7, 2003·Annals of Internal Medicine·Patrick M BossuytUNKNOWN Standards for Reporting of Diagnostic Accuracy
Sep 29, 2011·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·B BuyankhishigH L Rieder

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Citations

Nov 6, 2013·The American Journal of Tropical Medicine and Hygiene·Ali Akbar VelayatiSven Hoffner
Oct 3, 2017·Biomédica : revista del Instituto Nacional de Salud·Nelson José Alvis-ZakzukJosé Mauricio Hernández
Apr 22, 2020·Antimicrobial Agents and Chemotherapy·Erdenegerel NarmandakhChen-Yuan Chiang
Nov 17, 2016·International Journal of Mycobacteriology·Mohanad M AhmedSuhad H Mohammed
Nov 13, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Saba KabirAbdul Rehman

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