Rapid monitoring of cerebral ischemia dynamics using laser-based optical imaging of blood oxygenation and flow.

Biomedical Optics Express
Hart LevyOfer Levi

Abstract

Imaging blood flow or oxygenation changes using optical techniques is useful for monitoring cortical activity in healthy subjects as well as in diseased states such as stroke or epilepsy. However, in order to gain a better understanding of hemodynamics in conscious, freely moving animals, these techniques must be implemented in a small scale, portable design that is adaptable to a wearable format. We demonstrate a novel system which combines the two techniques of laser speckle contrast imaging and intrinsic optical signal imaging simultaneously, using compact laser sources, to monitor induced cortical ischemia in a full field format with high temporal acquisition rates. We further demonstrate the advantages of using combined measurements of speckle contrast and oxygenation to establish absolute flow velocities, as well as to statistically distinguish between veins and arteries. We accomplish this system using coherence reduction techniques applied to Vertical Cavity Surface Emitting Lasers (VCSELs) operating at 680, 795 and 850 nm. This system uses minimal optical components and can easily be adapted into a portable format for continuous monitoring of cortical hemodynamics.

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Citations

Feb 13, 2013·Applied Optics·Yaohai LinDanhua Liu
Jan 28, 2014·Biomedical Optics Express·Iliya SigalOfer Levi
Nov 27, 2014·Biomedical Optics Express·Inbar YarivDror Fixler
Mar 3, 2015·Journal of Biophotonics·Inbar YarivDror Fixler
Jun 10, 2014·Physiological Measurement·John Allen, Kevin Howell
Nov 22, 2018·Biomedical Optics Express·Dene RinguetteOfer Levi

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Methods Mentioned

BETA
imaging techniques
fluorescence imaging

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