Rapid monitoring of intermediate states and mass balance of nitrogen during denitrification by means of cavity enhanced Raman multi-gas sensing

Analytica Chimica Acta
Robert KeinerTorsten Frosch

Abstract

The comprehensive investigation of changes in N cycling has been challenging so far due to difficulties with measuring gases such as N2 and N2O simultaneously. In this study we introduce cavity enhanced Raman gas spectroscopy as a new analytical methodology for tracing the stepwise reduction of (15)N-labelled nitrate by the denitrifying bacteria Pseudomonas stutzeri. The unique capabilities of Raman multi-gas analysis enabled real-time, continuous, and non-consumptive quantification of the relevant gases ((14)N2, (14)N2O, O2, and CO2) and to trace the fate of (15)N-labeled nitrate substrate ((15)N2, (15)N2O) added to a P. stutzeri culture with one single measurement. Using this new methodology, we could quantify the kinetics of the formation and degradation for all gaseous compounds (educts and products) and thus study the reaction orders. The gas quantification was complemented with the analysis of nitrate and nitrite concentrations for the online monitoring of the total nitrogen element budget. The simultaneous quantification of all gases also enabled the contactless and sterile online acquisition of the pH changes in the P. stutzeri culture by the stoichiometry of the redox reactions during denitrification and the CO2-bicarb...Continue Reading

References

Mar 1, 1982·Microbiological Reviews·R Knowles
Feb 24, 2001·Nursing Standard·R Sander
Oct 3, 2002·Biochimica Et Biophysica Acta·Laurent Philippot
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Jorge LalucatNorberto J Palleroni
Jan 30, 2007·The Journal of Physical Chemistry. B·Torsten FroschJürgen Popp
Mar 31, 2007·The Journal of Physical Chemistry. B·Torsten FroschJürgen Popp
Jul 14, 2007·Analytical Chemistry·Torsten FroschJürgen Popp
Aug 28, 2007·The Journal of Physical Chemistry. B·Torsten FroschJürgen Popp
Jun 19, 2008·FEMS Microbiology Ecology·Marco ReicheKirsten Küsel
May 5, 1995·Biotechnology and Bioengineering·J S AlmeidaM J Carrondo
May 29, 2009·The Analyst·Torsten FroschJürgen Popp
Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anja KampPeter Stief
Mar 13, 2012·FEMS Microbiology Ecology·Anke HädrichKirsten Küsel
Sep 29, 2012·Environmental Microbiology·Martina HerrmannKirsten Küsel
Jun 14, 2013·Analytical Chemistry·Torsten FroschJürgen Popp
Jan 13, 2015·Analytical and Bioanalytical Chemistry·Robert KeinerTorsten Frosch

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Citations

Sep 8, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Timea FroschTorsten Frosch
Dec 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Timea FroschTorsten Frosch
Sep 19, 2019·Analytical and Bioanalytical Chemistry·Anne SieburgTorsten Frosch
Nov 22, 2016·Applied Optics·Dmitry V Petrov
Apr 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Domes ChristianTorsten Frosch
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sebastian WolfTorsten Frosch
Jul 24, 2021·Analytical and Bioanalytical Chemistry·Annika BlohmTorsten Frosch
Nov 1, 2017·Analytical Chemistry·Di YanTorsten Frosch
Aug 28, 2020·Analytical Chemistry·Andreas KneblTorsten Frosch
Nov 6, 2018·Analytical Chemistry·Di YanTorsten Frosch
May 6, 2019·Analytical Chemistry·Andreas KneblTorsten Frosch

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