Rapid multiplex PCR assays in patients with respiratory viral infections: is semi-quantitative data useful? A pilot study.

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Pierre GazeauLéa Pilorgé

Abstract

Viral respiratory rapid multiplex PCR assays FilmArray® (FA) and ePlex® (eP) provide qualitative results which may not reflect clinical relevance. In a pilot study, we report retrospectively whether the semi-quantitative PCR assay R-GENE® would have facilitated clinical interpretation. Forty-four patients were hospitalized for various respiratory manifestations; all of them have benefited from a respiratory sample during acute symptoms. Among the 44 patients, FA detected 23 positive samples including 31 viruses, 26 of them gave high or moderate R-GENE® scores (cycle threshold < 35), and all but one were consistent with clinical history. Semi-quantitative scores would have allowed for critical interpretation of the results; those are a key additional element for an optimal exploitation of the rapid multiplex PCR assays power.

References

Apr 10, 2010·The Journal of Infectious Diseases·Lincoln L H LauGabriel M Leung
Jun 15, 2011·Influenza and Other Respiratory Viruses·Emily T MartinJanet A Englund
Feb 26, 2014·Pediatrics·Samuel RhedinPontus Naucler
Aug 26, 2014·Archives of Pathology & Laboratory Medicine·Beverly B RogersJames E Bost
Mar 13, 2015·Journal of Clinical Microbiology·Paul C Schreckenberger, Alexander J McAdam
Apr 21, 2016·PloS One·Miriam Cebey-LópezUNKNOWN GENDRES network
Nov 29, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Andrea GranadosJonathan B Gubbay
Dec 7, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·H-S HuangC-C Lee

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