Rapid nanoscale quantitative analysis of plant sphingolipid long-chain bases by GC-MS

Analytical and Bioanalytical Chemistry
Jean-Luc CacasSébastien Mongrand

Abstract

In eukaryotic organisms, sphingolipids are major structural lipids of biological membranes and perform additional essential functions as signalling molecules. While long-chain bases (LCB), the common precursor to all sphingolipid classes, is represented by only one major molecular species in animals and fungi, up to nine LCB have been found in plants. In the absence of genuine plant sphingolipid references required for proper quantification, we have reinvestigated and optimized a protocol destined to the quantification of total plant LCB that relies on the use of gas chromatography-mass spectrometry (GC-MS). This rapid three-step protocol sequentially involves (1) the release of LCB from biological samples using barium hydroxide solution, (2) their oxidation into aldehydes by metaperiodate, and (3) the subsequent identification/quantification of these aldehydes by GC-MS. It is simple and reliable and enables separation of aldehydes upon their stero-specificity. It further enables the quantification of total LCB from a wide variety of samples including yeast and animal cell cultures.

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Citations

May 3, 2013·Lipids·Magdalena A Karaś, Ryszard Russa
Nov 13, 2013·Analytical Chemistry·Min LiHuwei Liu
Jul 28, 2013·Analytical and Bioanalytical Chemistry·Corinne BuréJean-Marie Schmitter
Aug 16, 2015·Journal of Chromatography. a·Núria CanelaLluís Arola
Apr 5, 2016·Journal of Mass Spectrometry : JMS·Corinne BuréJean-Marie Schmitter
May 6, 2016·Frontiers in Microbiology·Ashutosh Singh, Maurizio Del Poeta
Jul 7, 2015·Analytical Chemistry·Fabian AichelerOliver Kohlbacher
Sep 10, 2016·Journal of Analytical Methods in Chemistry·Lígia PimentelLuis Miguel Rodríguez-Alcalá
Nov 3, 2016·Frontiers in Plant Science·Antoine De Bigault Du Granrut, Jean-Luc Cacas
Nov 28, 2019·International Journal of Molecular Sciences·Enrica TorrettaCecilia Gelfi

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