Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.

Antimicrobial Agents and Chemotherapy
R Sanchez-PescadorM S Urdea

Abstract

A novel nucleic acid assay has been developed to screen bacterial populations for the presence of the tetM structural gene. The method involves the specific hybridization of several synthetic oligonucleotides to the gene in a crude bacterial lysate solution. As few as 1.5 x 10(4) CFU can be detected with the assay.

References

Dec 25, 1976·Lancet·A PercivalE H Annels
Jul 1, 1985·Antimicrobial Agents and Chemotherapy·M C RobertsG E Kenny
Feb 1, 1986·Antimicrobial Agents and Chemotherapy·M C Roberts, G E Kenny
Nov 1, 1986·Antimicrobial Agents and Chemotherapy·M C RobertsG E Kenny
Jan 1, 1984·Annual Review of Microbiology·J G Cannon, P F Sparling
Jun 1, 1963·Journal of Bacteriology·D S KELLOGGD I PIRKLE

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Citations

Nov 1, 1990·Analytical Chemistry·S Beck, H Köster

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