PMID: 11337136May 5, 2001Paper

Rapid pathotyping of Newcastle disease virus (NDV) using fluorogenic probes in a PCR assay

Veterinary Microbiology
E W AldousD J Alexander

Abstract

Hybridisation of PCR fragments with fluorogenic probes specific for pathotype allowed an estimation of pathogenicity of Newcastle disease virus (NDV) isolates using a modified TaqMan procedure. Six probes were used, designed to recognise nucleotide sequences in the fusion protein gene sequence corresponding to the precursor protein F0 cleavage site of both virulent and avirulent viruses. Forty-three of the 45 isolates tested, including 18 examined in a blind study were pathotyped successfully and rapidly, with close correlation between cleavage site nucleotide sequences, TaqMan results and intracerebral pathogenicity index (ICPI) values. One isolate, which could not be pathotyped by nucleotide sequencing, was shown using the TaqMan system to be a mixture of virulent and avirulent NDV. The results of this study suggest that using this modified TaqMan protocol, the likely virulence of most ND isolates can be determined rapidly and reproducibly.

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Citations

Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·Aman KumarNarender Singh Maan
Oct 1, 2001·Avian Pathology : Journal of the W.V.P.A·K Yusoff, W S Tan
Jul 28, 2012·Avian Pathology : Journal of the W.V.P.A·Dennis J AlexanderChad M Fuller
Sep 18, 2012·Tropical Animal Health and Production·Adriano de Oliveira Torres CarrascoAramis Augusto Pinto
Nov 30, 2006·Journal of Virological Methods·Hualei LiuTiangang Xu
Jul 17, 2012·Journal of Virological Methods·Oliver LungDirk Deregt
Nov 28, 2012·Journal of Virological Methods·Cristian De BattistiGiovanni Cattoli
Mar 11, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I M Mackay
Apr 1, 2001·Avian Pathology : Journal of the W.V.P.A·E W Aldous, D J Alexander
Sep 13, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Giovanni CattoliCorrie Brown
Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·L Mia KimDavid L Suarez
Apr 14, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Beate M CrossleyAlex A Ardans
Mar 21, 2002·Expert Review of Molecular Diagnostics·S Belák, P Thorén
Mar 3, 2004·BioTechniques·Priadarishni RamanujamKhatijah Yusoff
Sep 4, 2018·BioMed Research International·Muhammad Bashir BelloAbdul Rahman Omar
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia

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