Rapid PCR-based delineation of the porcine nodular worms, Oesophagostomum dentatum and O. quadrispinulatum

Molecular and Cellular Probes
L A NewtonR B Gasser

Abstract

At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed to overcome this limitation. The ITS-2 sequence of each species was determined, and specific oligonucleotide primers were designed to regions of greatest sequence difference between the species. Utilizing these primers, rapid PCR procedures were developed for the specific amplification of DNA of O. dentatum or O. quadrispinulatum, which are now used routinely to monitor the purity of larval cultures and to confirm the identity of larvae derived from the intestine or faeces. The application of specific PCR has major implications for studying the population biology of nodular worms in the pig model.

References

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May 25, 1993·Nucleic Acids Research·R B GasserI Beveridge
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Dec 1, 1995·Parasitology Today·A M Polderman, J Blotkamp
Jul 1, 1989·Parasitology Today·T B Stewart, L C Gasbarre

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Citations

Sep 10, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Cécile PatrelleDamien Jouet
Aug 11, 2020·Journal of Animal Science·Charlotte GaillardJean-Yves Dourmad
Feb 19, 1998·International Journal for Parasitology·R B Gasser
Mar 25, 2000·International Journal for Parasitology·R B Gasser, S E Newton
Aug 24, 1999·Veterinary Parasitology·R B Gasser

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