PMID: 45184Jan 1, 1979

Rapid pH and deltamuH+ jump by short laser pulse

Journal of Biochemical and Biophysical Methods
M Gutman, D Huppert

Abstract

The excited state (S1) of sulfononaphthols has a pK value well below that of the ground state, consequently intensive illumination of their aqueous solution should lead to acidification. In this study the second harmonies of a ruby laser pulse (10 MW, 30 ns at 347.2 nm) were used for excitation of a sulfononaphthol solution, resulting in a lowering of the pH from 8 to 4. The change in pH was demonstrated by spectral changes of the pH indicators Bromocresol Green, Bromothymol Blue, Bromocresol Purple and Phenol Red. The magnitude of the pH change was calculated from the kinetics of the changes in the indicators' absorbance and from fluorescence intensity of naphtholate. Sulfononaphthols, due to their hydrophylic nature, cannot permeate across phospholipid membranes. Taking advantage of this property, liposomes containing sulfononaphthol were prepared and irradiated by the laser pulse. Evidence is given that under such conditions the change in pH was limited to the space enclosed in the liposomes. The resulting proton-motive force (deltamuH+ = 180 -- 240 mV) is adequate for perturbing the energy-coupled reactions of oxidative phosphorylation. Possible applications of this technique in chemical physics, chemistry, biochemistry and...Continue Reading

References

Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell

Citations

Dec 1, 2005·Journal of Biological Physics·R FriedmanM Gutman
Jan 1, 1997·Annual Review of Physical Chemistry·M Gutman, E Nachliel
Jan 10, 2013·Annual Review of Physical Chemistry·Arnulf RosspeintnerEric Vauthey
May 17, 2005·Biophysical Journal·R FriedmanM Gutman
Aug 22, 2001·Biochimica Et Biophysica Acta·E Nachliel, M Gutman
Aug 9, 2016·The Journal of Physical Chemistry. B·Thomas T EisenhartJillian L Dempsey
Jun 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M GutmanR Friedman
Mar 1, 1983·European Journal of Biochemistry·H J GrandeC Veeger
Jul 15, 1983·European Journal of Biochemistry·M GutmanR Giniger
Feb 11, 2020·Macromolecular Rapid Communications·Felix WendlerFelix H Schacher
Jul 31, 2003·Journal of Clinical Periodontology·P BullonM Battino
Apr 3, 2004·British Journal of Haematology·João Paulo Vieira Pinheiro, Joachim Boos
Apr 29, 2016·Chembiochem : a European Journal of Chemical Biology·Oliver S ShafaatDennis A Dougherty

Related Concepts

2-naphthol-3,6-disulfonic acid, sodium salt
2-naphthol-6-sulfonic acid, potassium salt
Hydrogen-Ion Concentration
Masers
Niosomes
Study, Methodological
Naphthalenesulfonates
Oscillometry
Phosphatidylcholines
Photodegradation

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