Rapid phenotyping of cancer stem cells using multichannel nanosensor arrays

Nanomedicine : Nanotechnology, Biology, and Medicine
Yingying GengVincent M Rotello

Abstract

Cancer stem cells (CSCs) contribute to multidrug resistance, tumor recurrence and metastasis, making them prime therapeutic targets. Their ability to differentiate and lose stem cell properties makes them challenging to study. Currently, there is no simple assay that can quickly capture and trace the dynamic phenotypic changes on the CSC surface. Here, we report rapid discrimination of breast CSCs from non-CSCs using a nanoparticle-fluorescent-protein based sensor. This nanosensor was employed to discriminate CSCs from non-CSCs, as well as CSCs that had differentiated in vitro in two breast cancer models. Importantly, the sensor platform could also discriminate CSCs from the bulk population of cells in patient-derived xenografts of human breast cancer. Taken together, the results obtained demonstrate the feasibility of using the nanosensor to phenotype CSCs and monitor their fate. Furthermore, this approach provides a novel area for therapeutic interventions against these challenging targets.

Citations

Sep 20, 2019·Chemical Communications : Chem Comm·Ngoc D B LeVincent M Rotello
Feb 13, 2020·Chemical Communications : Chem Comm·Meng-Xian LiuJian-Hua Wang
Apr 7, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroka SugaiRyoji Kurita
Aug 21, 2019·Life Sciences·Masoud NajafiJamal Majidpoor
Jun 18, 2021·Journal of Molecular Recognition : JMR·Yuanyuan ShenYi Wan
Jun 15, 2021·Chemical Science·Yingying GengVincent M Rotello
Nov 10, 2020·ACS Nano·Yingying GengVincent M Rotello

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