Rapid Point-of-Care Isothermal Amplification Assay for the Detection of Malaria without Nucleic Acid Purification

Infectious Diseases
Sayli S ModakYhombi Serge Yvon Ongagna

Abstract

Malaria remains one of the most prevalent infectious diseases and results in significant mortality. Isothermal amplification (loop-mediated isothermal amplification) is used to detect malarial DNA at levels of ~1 parasite/µL blood in ≤30 minutes without the isolation of parasite nucleic acid from subject's blood or saliva. The technique targets the mitochondrial cytochrome oxidase subunit 1 gene and is capable of distinguishing Plasmodium falciparum from Plasmodium vivax. Malarial diagnosis by the gold standard microscopic examination of blood smears is generally carried out only after moderate-to-severe symptoms appear. Rapid diagnostic antigen tests are available but generally require infection levels in the range of 200-2,000 parasites/µL for a positive diagnosis and cannot distinguish if the disease has been cleared due to the persistence of circulating antigen. This study describes a rapid and simple molecular assay to detect malarial genes directly from whole blood or saliva without DNA isolation.

References

Mar 1, 1996·Applied and Environmental Microbiology·C A Kreader
Feb 12, 1998·BioTechniques·A J Sandford, P D Paré
Mar 21, 1998·Biochemistry and Molecular Biology International·M NagaiN Sato
Dec 2, 2000·Journal of Clinical Microbiology·W Abu Al-Soud, P Rådström
Jan 24, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J DolezelJ Greilhuber
Feb 16, 2007·Trends in Parasitology·Georges Snounou, Laurent Rénia
Feb 5, 2009·Lab on a Chip·Jitae KimHaim H Bau
Jun 18, 2010·Journal of Clinical Microbiology·Spencer D PolleyColin J Sutherland
Jul 14, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rochelle P WalenskyUNKNOWN CEPAC Investigators
Dec 2, 2010·Current Genomics·Jianbing MuXin-Zhuan Su
Jul 8, 2011·Lab on a Chip·Changchun LiuHaim H Bau
Oct 18, 2012·Genetic Testing and Molecular Biomarkers·Jianjie ZhuWeipeng Wang
Dec 20, 2013·Nature·Frédéric ArieyDidier Ménard

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Citations

Feb 6, 2019·European Journal of Microbiology & Immunology·Hans KollendaHagen Frickmann
Oct 3, 2019·Biosensors·Faye M Walker, Kuangwen Hsieh
Jan 22, 2021·Expert Review of Molecular Diagnostics·Taylor J MoehlingRobert J Meagher
Jul 14, 2018·Analytical Chemistry·Soroush KahkeshaniDino Di Carlo

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Datasets Mentioned

BETA
AB570973.1

Methods Mentioned

BETA
PCR
Protein Assay
RNA Assay
electrophoresis
nucleic acid amplification

Software Mentioned

Genie Explorer

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Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.