PMID: 3766942Aug 1, 1986Paper

Rapid preparation of bacteriophage DNA for sequence analysis in sets of 96 clones, using filtration

Analytical Biochemistry
I Eperon

Abstract

A method is described for the preparation of single-stranded DNA from clones in bacteriophage M13 vectors. This procedure allows multiples of 96 clones to be processed at once, utilizing filtration to remove host cells and simplifying the treatment of bacteriophage pellets. The DNA produced can be used for sequencing of mutagenesis.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Jul 10, 1981·Nucleic Acids Research·S Anderson
Jan 25, 1983·Nucleic Acids Research·W M Barnes, M Bevan
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P Abarzúa, K J Marians
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong

❮ Previous
Next ❯

Citations

Jan 25, 1988·Nucleic Acids Research·A D Turnbull-RossI C Eperon
Jun 25, 1991·Nucleic Acids Research·M Chee
Sep 11, 1991·Nucleic Acids Research·W D RawlinsonB G Barrell
Dec 15, 1996·Nucleic Acids Research·W L NgL Hood
Jan 1, 1994·DNA Sequence : the Journal of DNA Sequencing and Mapping·D E KolnerL M Smith
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·A T BankierE Preddie
Jan 1, 1990·DNA Sequence : the Journal of DNA Sequencing and Mapping·V SmithB G Barrell
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·T Hawkins
Jan 5, 1990·Journal of Molecular Biology·A L LearI C Eperon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.