Rapid preparation of stable isotope labeled peptides that bind to target proteins by a phage library system

Journal of Biomolecular NMR
Yumiko MizukoshiIchio Shimada

Abstract

We have developed a system for directly isolating foreign peptides displayed on the N-terminus of the major coat protein of bacteriophage M13. The phage particle in this system is formed as a mixture of wild type and modified coat proteins. The N-terminal segment of the modified coat protein was mutated for chemical cleavage, in order to obtain the displayed peptide from the major coat protein. Using 13C, 15N- labeled medium, we introduced stable isotopes, 13C and/or 15N, into the coat proteins. The NMR spectra for the cleaved peptides from the phage particles could be recorded within a few days after the selection of the phage clone.

References

Jul 27, 1990·Science·J K Scott, G P Smith
Nov 12, 1991·Biochemistry·H B LowmanJ A Wells
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S E CwirlaW J Dower
Dec 1, 1995·The Journal of Experimental Medicine·N KayagakiH Yagita
Jan 1, 1994·Methods in Enzymology·G M Clore, A M Gronenborn
Feb 12, 2000·Journal of Molecular Biology·S S SidhuJ A Wells
Feb 26, 2000·Science·W L DeLanoJ A Wells
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T NisiharaH Yagita
Apr 1, 1997·Chemical Reviews·George P. Smith, Valery A. Petrenko
Jan 4, 2003·Chembiochem : a European Journal of Chemical Biology·Sachdev S SidhuKurt Deshayes
May 27, 2003·Journal of Biomolecular NMR·Bernd W KoenigJohn M Louis

❮ Previous
Next ❯

Citations

Mar 17, 2010·Journal of Biomolecular NMR·Yumiko MizukoshiHideo Takahashi
Feb 21, 2009·The Journal of Biological Chemistry·Kozue Kato-TakagakiHideo Takahashi
Jan 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Toshihiko SugikiChojiro Kojima

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.