Rapid procedure for detection and isolation of large and small plasmids.

Journal of Bacteriology
C I Kado, S T Liu

Abstract

Procedures are described for the detection and isolation of plasmids of various sizes (2.6 to 350 megadaltons) that are harbored in species of Agrobacterium, Rhizobium, Escherichia, Salmonella, Erwinia, Pseudomonas, and Xanthomonas. The method utilized the molecular characteristics of covalently closed circular deoxyribonucleic acid (DNA) that is released from cells under conditions that denature chromosomal DNA by using alkaline sodium dodecyl sulfate (pH 12.6) at elevated temperatures. Proteins and cell debris were removed by extraction with phenol-chloroform. Under these conditions chromosomal DNA concentrations were reduced or eliminated. The clarified extract was used directly for electrophoretic analysis. These procedures also permitted the selective isolation of plasmid DNA that can be used directly in nick translation, restriction endonuclease analysis, transformation, and DNA cloning experiments.

Citations

Jan 1, 1992·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·D B Rajini Rani, A Mahadevan
Aug 1, 1992·The Journal of Applied Bacteriology·M T Ogan
Sep 1, 1992·Letters in Applied Microbiology·C S JonesJ Stanley
Aug 1, 1990·Epidemiology and Infection·A AgodiM Marranzano
Jan 1, 1990·Microbiology and Immunology·J YamamotoM Shimizu
Jan 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C S Goodwin, J A Armstrong
Sep 1, 1989·Oral Microbiology and Immunology·A Progulske-FoxW P McArthur
Aug 1, 1989·Molecular & General Genetics : MGG·S G SedgwickP Strike
May 1, 1988·Molecular Microbiology·V BonnefoyM Chippaux
Jun 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S M OpalL W Lyhte
Jan 1, 1987·Microbiology and Immunology·A MochizukiR Nakaya
Jan 1, 1988·Microbiology and Immunology·K TakahashiN Okamura
Sep 1, 1987·European Journal of Epidemiology·M Castellani PastorisC Passi
Jan 1, 1985·Microbiology and Immunology·K K KalaimatheeY F Ngeow
Jan 1, 1984·Molecular & General Genetics : MGG·M S Thomas, W T Drabble
Jan 1, 1983·Molecular & General Genetics : MGG·W KlingmüllerM Singh
Jan 1, 1982·Molecular & General Genetics : MGG·R C TaitC I Kado
Dec 1, 1984·European Journal of Clinical Microbiology·G E HollickA Spellacy
May 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A A Shehabi
Oct 1, 1995·The Journal of Applied Bacteriology·T TiainenJ L Larsen
Jan 1, 1994·Scandinavian Journal of Infectious Diseases·B OlesenI Orskov
Nov 15, 1994·Molecular & General Genetics : MGG·T P LinS T Liu
Nov 1, 1993·The Journal of Applied Bacteriology·E TorreB Rowe
Dec 1, 1993·Epidemiology and Infection·M SynnottJ Cowden
May 1, 1993·Journal of Periodontal Research·J D HillmanS S Socransky
Jan 1, 1997·Scandinavian Journal of Infectious Diseases·A R Bahrmand, A A Velayati
Aug 6, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·T DjajakusumahE Van Dyck
Mar 11, 2000·Microbiology and Immunology·Y InoshimaH Sentsui

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