Rapid procedure for the detection of plasmids in Staphylococcus epidermidis.

Applied and Environmental Microbiology
C R WilsonJ N Baldwin

Abstract

A rapid, reproducible, mini-volume assay capable of detecting staphylococcal plasmid DNA in the range of 0.8 to 32 megadaltons has been developed. The assay employs lysostaphin-mediated lysis of cells followed by a short, low-speed centrifugation and does not require treatment with ribonuclease or protease or deproteinization with phenol. A period of only 24 h may be required to detect the presence and size of a plasmid once an organism has been isolated. This method has been used to study the plasmid ecology of Staphylococcus epidermidis and to correlate the presence or absence of plasmids with tetracycline, chloramphenicol, neomycin, penicillin, and cadmium resistances.

References

Jan 28, 1977·Science·J TelfordM Birnstiel
Mar 1, 1975·Journal of Clinical Microbiology·K H Schleifer, W E Kloos
May 1, 1973·Antimicrobial Agents and Chemotherapy·B H Minshew, E D Rosenblum
Jun 11, 1971·Annals of the New York Academy of Sciences·R P Novick, D Bouanchaud
Oct 1, 1969·Applied Microbiology·J N BaldwinM F Cox

❮ Previous
Next ❯

Citations

Oct 1, 1986·The Journal of Hygiene·J A Melo CristinoJ N Naidoo
Jun 1, 1981·The Journal of Dairy Research·F L Davies, M J Gasson
Mar 1, 1991·The Journal of Applied Bacteriology·M RahmanW C Noble
Apr 1, 1986·The Journal of Applied Bacteriology·J K Thompson, A J Holding
Feb 1, 1988·The Journal of Applied Bacteriology·W C NobleD H Lloyd
Sep 1, 1981·Antimicrobial Agents and Chemotherapy·P A TottenJ N Baldwin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

American Journal of Veterinary Research
G H LuginbuhlD G Simmons
The Australian Journal of Experimental Biology and Medical Science
T V Riley, B J Mee
American Journal of Veterinary Research
T J McDonald, G W Pugh
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
M K VartanianR A Zakharian
© 2021 Meta ULC. All rights reserved