Rapid Prototyping Platform for Saccharomyces cerevisiae Using Computer-Aided Genetic Design Enabled by Parallel Software and Workcell Platform Development

SLAS Technology
P D RajakumarP S Freemont

Abstract

Biofoundries have enabled the ability to automate the construction of genetic constructs using computer-aided design. In this study, we have developed the methodology required to abstract and automate the construction of yeast-compatible designs. We demonstrate the use of our in-house software tool, AMOS, to coordinate with design software, JMP, and robotic liquid handling platforms to successfully manage the construction of a library of 88 yeast expression plasmids. In this proof-of-principle study, we used three fluorescent genes as proxy for three enzyme coding sequences. Our platform has been designed to quickly iterate around a design cycle of four protein coding sequences per plasmid, with larger numbers possible with multiplexed genome integrations in Saccharomyces cerevisiae. This work highlights how developing scalable new biotechnology applications requires a close integration between software development, liquid handling robotics, and protocol development.

References

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Citations

Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Richard J R KelwickPaul S Freemont
Jan 1, 2021·ACS Synthetic Biology·Guillermo Yáñez FeliúTimothy J Rudge
Jan 8, 2021·ACS Synthetic Biology·James GilmanFilippo Menolascina

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Methods Mentioned

BETA
flow cytometry
Fluorescence

Software Mentioned

CybioFelix
BioBlocks
PostgreSQL
BioCoder
Cello
AquaCore
MoClo Yeast Toolkit ( YTK )
AMOS
cerevisiae
LIMS ( Laboratory Information Management System )

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