Rapid quantification of aortic lesions in apoE(-/-) mice

Journal of Vascular Research
John H BeattieMargaret-Jane Gordon

Abstract

The quantification of aortic lesions is an important end-point analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification.

Citations

Nov 27, 2014·Journal of Translational Medicine·Junwen ChenShu Meng
Feb 15, 2018·International Journal of Molecular Sciences·Speranza RubattuMassimo Volpe
May 22, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hao ZhouYuandong Peng
May 18, 2017·Cardiovascular Research·Barbara Kutryb-ZajacRyszard T Smolenski
Jan 27, 2019·International Journal of Molecular Sciences·Michela ZanettiRocco Barazzoni
Jun 6, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aoshuang ChenGuoxing Zheng
Apr 30, 2020·Cell Death and Differentiation·Jung Hee ChoJae-Ryong Kim

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