Abstract
The quantification of aortic lesions is an important end-point analysis for evaluating atherogenesis in mouse models of atherosclerosis. Morphometric methods involving the staining of aorta with a Sudan lysochrome followed by image analysis of the stained lesion area are commonly used. We have developed a more rapid method involving solubilisation of the stain retained by aortic lesions. In 2 separate studies, 5-week-old male apoE(-/-) and C57BL/6 wild-type (apoE(+/+)) mice were given a high fat (21%), Western-type diet for 13, 15 or 25 weeks. At study termination, the descending thoracic aorta (DA) and/or aortic arch (AA) were stained with Oil Red O (ORO). The incorporated stain was extracted using chloroform/methanol (2:1) solvent and quantified by spectrophotometry at 520 nm. In study 1 (13 weeks), ORO stain in the AA and DA of apoE(-/-) mice was 1.9 and 1.4 times higher than background staining of apoE(+/+) aorta tissue, respectively. At 15 and 25 weeks (study 2), ORO stain in the AA of apoE(-/-) mice was 1.9 and 2.5 times higher than the background, respectively. We conclude that the ORO solubilisation technique applied to AA samples is a very useful and rapid method for atherosclerotic lesion quantification.
Citations
Nov 2, 2012·Molecular Medicine·Zdenka DjuricPeter P Nawroth
Apr 17, 2013·Drug Design, Development and Therapy·Yong ZhangXiaohua Fu
Nov 27, 2014·Journal of Translational Medicine·Junwen ChenShu Meng
Jun 19, 2012·Clinical Epigenetics·Chris J McNeilSusan J Duthie
Jan 1, 2016·Cell Death & Disease·R StanzioneS Rubattu
Dec 3, 2014·Genes & Nutrition·Susan J DuthieChristopher J McNeil
Dec 3, 2016·Nucleosides, Nucleotides & Nucleic Acids·Magdalena ZabielskaRyszard Smoleński
Feb 15, 2018·International Journal of Molecular Sciences·Speranza RubattuMassimo Volpe
Jul 5, 2012·Molecular Nutrition & Food Research·John H BeattieIn-Sook Kwun
Mar 8, 2012·Molecular Oral Microbiology·H-R LeeB-K Choi
May 23, 2012·Molecular Nutrition & Food Research·Christopher J McNeilSusan J Duthie
Jan 1, 2014·Oral Diseases·H R LeeB K Choi
May 22, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hao ZhouYuandong Peng
Apr 10, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Hekmat B KhoukazWilliam P Fay
May 18, 2017·Cardiovascular Research·Barbara Kutryb-ZajacRyszard T Smolenski
Jun 3, 2018·Cardiovascular Toxicology·Li-Jun ZhaoY James Kang
Jan 27, 2019·International Journal of Molecular Sciences·Michela ZanettiRocco Barazzoni
Jun 6, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aoshuang ChenGuoxing Zheng
Apr 30, 2020·Cell Death and Differentiation·Jung Hee ChoJae-Ryong Kim
Sep 12, 2015·Oncotarget·Mariateresa MancusoAnna Saran
Jun 14, 2018·Frontiers in Immunology·Fei ShuYan Shi
May 8, 2021·Frontiers in Medicine·Marcela Frota CavalcanteDulcineia Saes Parra Abdalla