Rapid quantification of periodontitis-related bacteria using a novel modification of Invader PLUS technologies

Microbiological Research
Kenichi TadokoroHirokazu Oguchi

Abstract

The Invader PLUS technology is a sensitive, rapid method for the detection and quantification of nucleic acid. While the original technology is based on the amplification by polymerase chain reaction (PCR) of the target sequence followed by its detection using the Invader technology, the current modification allows simultaneous PCR amplification and Invader reaction. The PCR primers and the Invader probes are designed to operate at the same temperature. This allows simpler design and faster results. This technology has been applied for the quantification of six periodontitis-related bacteria (Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Toreponema denticola, Tannerella forsythensis and Fusobacterium nucleatum). Direct comparison of this modified Invader PLUS with real-time PCR demonstrated similar linear range. Furthermore, testing of 64 volunteers showed a good correlation between both technologies with correlation factors r2 spanning between 0.827 and 0.987. We demonstrated here that the proposed improvement of the Invader PLUS allows the detection and quantification of DNA sequences using a simple design and protocol that can be implemented in clinical testing.

References

Mar 29, 2001·Microbiology and Immunology·M SakamotoY Benno
Aug 25, 2004·Journal of Microbiological Methods·Claudia NonnenmacherKlaus Heeg
Feb 4, 2006·Journal of Clinical Microbiology·Jeffrey J GermerJoseph D C Yao

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Citations

Oct 12, 2016·Journal of Oral Science·Yorimasa OgataHiromasa Yoshie
May 2, 2017·Photodiagnosis and Photodynamic Therapy·Yoshiaki NomuraNobuhiro Hanada
Dec 6, 2017·PloS One·Yoshiaki NomuraHiromasa Yoshie
Sep 22, 2012·Japanese Journal of Infectious Diseases·Akio TadaNobuhiro Hanada
Jul 16, 2019·Immunity, Inflammation and Disease·Megumi IwauchiShouji Hironaka
Oct 22, 2019·Biomedical Reports·Yumiko Nagao, Takeshi Tanigawa
Jun 1, 2011·Australian Dental Journal·J SatoT Arai

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