Rapid Quantification of SARS-CoV-2-Neutralizing Antibodies Using Propagation-Defective Vesicular Stomatitis Virus Pseudotypes.

Vaccines
Ferdinand ZettlGert Zimmer

Abstract

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2, a new member of the genus Betacoronavirus, is a pandemic virus, which has caused numerous fatalities, particularly in the elderly and persons with underlying morbidities. At present, there are no approved vaccines nor antiviral therapies available. The detection and quantification of SARS-CoV-2-neutralizing antibodies plays a crucial role in the assessment of the immune status of convalescent COVID-19 patients, evaluation of recombinant therapeutic antibodies, and the evaluation of novel vaccines. To detect SARS-CoV-2-neutralizing antibodies, classically, a virus-neutralization test has to be performed at biosafety level 3, considerably limiting the general use of this test. In the present work, a biosafety level 1 pseudotype virus assay based on a propagation-incompetent vesicular stomatitis virus (VSV) has been used to determine the neutralizing antibody titers in convalescent COVID-19 patients. The neutralization titers in serum of two independently analyzed patient cohorts were available within 18 h and correlated well with those obtained with a classical SARS-CoV-2 neutralization test (Pearson correlation coefficients of r = 0.929 and r = 0.939, respectively...Continue Reading

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Citations

Aug 11, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Nina BabelTimm H Westhoff
Oct 13, 2020·Emerging Microbes & Infections·Benjamin MeyerChantal Reusken
Nov 13, 2020·Journal of the Pediatric Infectious Diseases Society·Serge GrazioliGeraldine Blanchard-Rohner
Feb 14, 2021·Science Advances·Carolina Beltrán-PavezRicardo Soto-Rifo
Jan 1, 2021·Vaccines·Lydia RieplerJanine Kimpel
Apr 13, 2021·Expert Review of Molecular Diagnostics·Jianfu J WangJin V Wu
Apr 30, 2021·Journal of Immunological Methods·Aria C Shi, Ping Ren
Apr 30, 2021·Frontiers in Cellular and Infection Microbiology·Obdulio García-NicolásArtur Summerfield
Aug 31, 2021·One Health·Carmen W E EmbregtsCorine H GeurtsvanKessel
Nov 2, 2021·Frontiers in Cellular and Infection Microbiology·Haijun TangF Xiao-Feng Qin

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Methods Mentioned

BETA
transgenic
enzyme-linked immunosorbent assays
ELISA
transfection

Software Mentioned

GraphPad

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