PMID: 9174279Apr 11, 1997Paper

Rapid quantitation of (-)-2'-deoxy-3'-thiacytidine in human serum by high-performance liquid chromatography with ultraviolet detection

Journal of Chromatography. B, Biomedical Sciences and Applications
X J Zhou, J P Sommadossi

Abstract

A rapid, sensitive and specific high-performance liquid chromatographic (HPLC) assay was developed and validated for the measurement of (-)-2'-deoxy-3'-thiacytidine (3TC) in human serum. The method included precipitation of serum proteins by trichloroacetic acid (20%, w/v) treatment followed by centrifugation. The resulting supernatant was directly injected and 3TC was isocratically chromatographed on a reversed-phase C18 column using a mixture of phosphate buffer and methanol (88.3:11.7. v/v) and monitored at 280 nm. The limit of quantitation was 20 ng/ml using 100 microl of serum. The standard curve was linear within the range of 20-10,000 ng/ml. Replicate analysis of three quality control samples (40-1500 ng/ml) led to satisfactory intra- and inter-assay precision (coefficient of variation from 3.0 to 12.9%) and accuracy (deviation from 6.3 to 9.7%). Moreover, sample treatment processes including human immunodeficiency virus (HIV) heat-inactivation, exposure at room temperature and freezing-thawing cycles did not influence the stability of the analyte. This assay was successfully applied to the determination of 3TC serum levels in HIV-infected patients. In addition, preliminary results indicated that this procedure may also ...Continue Reading

References

Dec 21, 1995·The New England Journal of Medicine·J L DienstagM Rubin
Oct 1, 1994·Journal of Clinical Pharmacology·E K HusseyG L Evans
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TisdaleB A Larder
Jul 1, 1994·Journal of Chromatography. B, Biomedical Applications·A J HarkerD M Morris
Feb 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·D M Morris, K Selinger

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Citations

Apr 6, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yazen AlnoutiMichael G Bartlett
Jul 28, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gholamreza BahramiBahareh Mohammadi
Jun 14, 2005·Journal of Pharmaceutical and Biomedical Analysis·Sockalingam AnbazhaganSeshaiah Krishnan Sridhar

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