Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens

Journal of Medical Virology
I EngelmannA Heim

Abstract

Rapid diagnosis of human herpesvirus primary infections or reactivations is facilitated by quantitative PCRs. Quantitative PCR assays with a standard thermal cycling profile permitting simultaneous detection of herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV6) DNA were developed and validated for diagnostic use. High specificity and sensitivity were achieved and the new PCR assays correlated well with commercial PCR assays. Twenty two thousand eight hundred sixty eight PCR tests were undertaken on specimens obtained from immunosuppressed patients. DNAemia was frequent with EBV (43.5%), HHV6 (32.4%), CMV (12.8%), and VZV (12.9%). As already described for EBV and CMV, high virus loads of HHV6 and VZV were associated with clinical symptoms and poor clinical outcome, for example, three of four patients with VZV virus loads >10(5) copies/ml died. A high proportion of lower respiratory specimens was positive for EBV- (38.8%), HHV6- (29.4%), and CMV-DNA (18.2%). For CMV, infection was confirmed in 66.7% of patients by virus isolation or positive pp65 antigenaemia. Differentiation of HHV6A, -B and HSV-1, -2 by melting curve analysis revealed that HH...Continue Reading

References

Feb 24, 1995·Cell·E M MiyashitaD A Thorley-Lawson
Dec 1, 1996·American Journal of Respiratory and Critical Care Medicine·R M ArisJ Handy
Nov 14, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P RautenbergD Franke
Aug 15, 1998·Journal of Clinical Microbiology·C Y TongA Bakran
Oct 13, 1998·Immunity·G J BabcockD A Thorley-Lawson
Apr 25, 2000·Respiration; International Review of Thoracic Diseases·B BewigA Haverich
Sep 2, 2000·Journal of Clinical Microbiology·M J EspyT F Smith
May 30, 2001·Lancet·R J Whitley, B Roizman
Dec 18, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Angela M KearnsRoger Freeman
Jan 12, 2002·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Barbara C GärtnerNikolaus Müller-Lantzsch
Mar 13, 2002·Transplantation·Atul HumarTony Mazzulli
Sep 25, 2003·European Journal of Clinical Nutrition·U Alexy, M Kersting
Feb 27, 2004·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Rokshana Shroff, Lesley Rees
Apr 10, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Růzena StránskáAnton M van Loon
May 20, 2005·Pathology, Research and Practice·Georg Werner HergetGabriele Köhler
Feb 24, 2006·American Journal of Clinical Dermatology·Hiok-Hee Tan, Chee-Leok Goh
Nov 30, 2006·Journal of Medical Virology·Hoe Nam LeongDuncan A Clark
Feb 13, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Nicolaas A BakkerWillem J van Son
Apr 10, 2007·Lancet·André SchrauderArmin Wessel

❮ Previous
Next ❯

Citations

Jun 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wouter H RoosGijs J L Wuite
Jun 30, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Uta ErdbrueggerWilfried Gwinner
Jan 13, 2010·Clinical Microbiology Reviews·D Scott Schmid, Aisha O Jumaan
Sep 24, 2010·Virology Journal·Meav-Lang J LayUNKNOWN Ausimmune Investigator Group (AIG)
Aug 7, 2012·Journal of Applied Physics·Anirban MitraLukas Novotny
Feb 9, 2011·Cutaneous and Ocular Toxicology·Maria João CruzFilomena Azevedo
Sep 15, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tina GanzenmuellerAlbert Heim
Mar 24, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ilka EngelmannJens Gottlieb
Feb 18, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L SchifferB Maecker-Kolhoff
Apr 30, 2013·Transplant International : Official Journal of the European Society for Organ Transplantation·An-Chieh FengHsiu-Lung Fan
Oct 3, 2012·Transplant Infectious Disease : an Official Journal of the Transplantation Society·E SchlischewskyJ Gottlieb
Mar 8, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J LeUNKNOWN AST Infectious Diseases Community of Practice
Dec 16, 2010·Transplant Infectious Disease : an Official Journal of the Transplantation Society·I EngelmannF Mattner
Dec 19, 2009·Periodontology 2000·Jørgen Slots
Nov 13, 2009·The Journal of General Virology·Charles CunninghamAndrew J Davison
Jan 24, 2015·Case Reports in Neurological Medicine·Thomas PasedagThomas Skripuletz
Dec 25, 2010·Revista Da Sociedade Brasileira De Medicina Tropical·Kátia Silene Sousa CarvalhoLuiz Felipe Leomil Coelho
Aug 11, 2012·Progress in Transplantation : Official Publication, North American Transplant Coordinators Organization ... [et Al.]·Frauke MattnerIlka Engelmann
May 29, 2018·BMC Infectious Diseases·Thomas SkripuletzCorinna Trebst
Jul 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Carina ElsnerChristine Goffinet
Sep 12, 2015·American Journal of Physiology. Renal Physiology·Uta ErdbrueggerWilfried Gwinner
Jun 24, 2016·Microbiology Spectrum·Henri AgutAgnès Gautheret-Dejean
Jun 24, 2016·Microbiology Spectrum·Myron J LevinD Scott Schmid
Dec 5, 2019··Alexander SteinkassererAlexandra Düthorn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

The New England Journal of Medicine
Leonardo PotenzaGiovanni Guaraldi
Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Masahiro OhashiTetsushi Yoshikawa
© 2021 Meta ULC. All rights reserved