Jul 1, 1975

Rapid radioimmunoassay for guanosine 3',5'-cyclic monophosphate using tritiated ligand

Journal of Biochemistry
M FujimotoS Tarui

Abstract

A radioimmunoassay procedure for guanosine 3',5'-cyclic monophosphate (CGMP) is described. The procedure is based on competitive binding between [3H]CGMP and non-radioactive CGMP, with separation of bound and unbound CGMP by Millipore filtration. The binding reaction showed very high specificity to CGMP, had a broad pH optimum, and reached equilibrium within a short time. A simple procedure for the pruification of assay samples using Dowex AG 50W-X2 resin is also described. CGMP contents in urine samples were assayed without purification. Injection of glucagon into healthy human volunteers resulted in a small but significant reduction in urinary CGMP level, whereas CAMP excretion increased dramatically.

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Mentioned in this Paper

Human Volunteers
Phosphoric diester hydrolase
Injection Procedure
Glucagon
Tritium
Cyclic AMP, (R)-Isomer
Excretory Function
Binding (Molecular Function)
Cyclic GMP-Dependent Protein Kinases
Millipore Filters

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