PMID: 2508202May 1, 1989Paper

Rapid radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis

Research in Microbiology
Max SalfingerZ T Johnson

Abstract

Pyrazinamide (PZA) is one of the most important drugs in modern chemotherapy of tuberculosis. Since PZA is active only at an acid pH, testing the susceptibility of Mycobacterium tuberculosis to PZA is difficult and timeconsuming. Therefore, we evaluated the BACTEC system for rapid testing of PZA susceptibility at pH 6. A total of 91 M. tuberculosis strains and 2 different strains of M. bovis BCG were screened for susceptibility to PZA. Each strain was tested in special 7H12 broth supplemented with polyoxyethylene stearate containing 25, 50 and 100 micrograms PZA/ml. Strains resistant to 100 micrograms/ml were retested against 25-100 micrograms/ml and at an extended range of PZA concentrations from 200-6,400 micrograms/ml. The MIC was determined with all strains within 4-20 (mean 7) days. Of the 77 susceptible strains, based on the pyrazinamidase test, MIC were less than or equal to 25 micrograms/ml for 34 strains, 50 for 38 and 100 for 2 strains. Three pyrazinamidase-positive strains had still higher MIC, 1 at 800 and 2 at 3,200 micrograms/ml. PZA-resistant strains had MIC of 800 or greater. Monoresistance to PZA has not been detected to date. The clear bimodal distribution of MIC in this method could enable the routine clinica...Continue Reading

References

Feb 1, 1989·The American Review of Respiratory Disease·A J CrowleM H May
Jul 1, 1988·Antimicrobial Agents and Chemotherapy·M Salfinger, L B Heifets
Nov 1, 1986·Contact Dermatitis·M R Holdiness
Sep 1, 1985·Tubercle·P A Jenkins
Jan 1, 1969·Radiology·F H DeLand, H N Wagner
Oct 1, 1983·Antimicrobial Agents and Chemotherapy·W R Butler, J O Kilburn
Jul 1, 1958·American Journal of Public Health and the Nation's Health·G MIDDLEBROOK, M L COHN

❮ Previous
Next ❯

Citations

Jul 1, 2002·Indian Journal of Clinical Biochemistry : IJCB·V K Vijayan
Dec 1, 1997·Indian Journal of Clinical Biochemistry : IJCB·L M SrivastavaR C Jain
Aug 1, 1994·International Journal of Antimicrobial Agents·V St Georgiev
Jun 19, 2013·Nature Chemical Biology·Regina WilsonDavid Alland
Feb 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·K Fuursted
Aug 22, 2007·Antimicrobial Agents and Chemotherapy·Emma HuitricSven E Hoffner
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Kwok Chiu ChangYing Zhang
Aug 1, 1983·Antimicrobial Agents and Chemotherapy·R T Nozawa, T Yokota
Jul 1, 1988·Antimicrobial Agents and Chemotherapy·M Salfinger, L B Heifets
Aug 1, 1988·Antimicrobial Agents and Chemotherapy·L E NilssonS Anséhn
Sep 1, 1989·Antimicrobial Agents and Chemotherapy·S Naik, R Ruck
Jun 1, 1990·Antimicrobial Agents and Chemotherapy·V BoonkitticharoenP T Kirchner
Oct 1, 1995·Antimicrobial Agents and Chemotherapy·G A ChungK Duncan
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·G P MorlockR C Cooksey
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Juan-Carlos PalominoFrançoise Portaels
Aug 17, 2002·Antimicrobial Agents and Chemotherapy·Vanja M BulatovicFrank Rusnak
Oct 1, 2010·Journal of Clinical Microbiology·Iván Hernández-NeutaPatricia Del Portillo
Sep 7, 2007·Journal of Clinical Microbiology·Elvira RichterJette E Kristiansen
Jun 8, 2006·Journal of Clinical Microbiology·Pontus JureenSven E Hoffner
Aug 30, 2001·Journal of Clinical Microbiology·A KrüünerG Källenius
Feb 5, 2002·Journal of Clinical Microbiology·K A Kristian AngebySven E Hoffner
May 1, 2002·Journal of Clinical Microbiology·Gaby E PfyfferSabine Rüsch-Gerdes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.