Rapid reagentless quantification of alginate biosynthesis in Pseudomonas fluorescens bacteria mutants using FT-IR spectroscopy coupled to multivariate partial least squares regression.

Analytical and Bioanalytical Chemistry
Elon CorreaRoyston Goodacre

Abstract

Alginate is an important medical and commercial product and currently is isolated from seaweeds. Certain microorganisms also produce alginate and these polymers have the potential to replace seaweed alginates in some applications, mainly because such production will allow much better and more reproducible control of critical qualitative polymer properties. The research conducted here presents the development of a new approach to this problem by analysing a transposon insertion mutant library constructed in an alginate-producing derivative of the Pseudomonas fluorescens strain SBW25. The procedure is based on the non-destructive and reagent-free method of Fourier transform infrared (FT-IR) spectroscopy which is used to generate a complex biochemical infrared fingerprint of the medium after bacterial growth. First, we investigate the potential differences caused by the growth media fructose and glycerol on the bacterial phenotype and alginate synthesis in 193 selected P. fluorescens mutants and show that clear phenotypic differences are observed in the infrared fingerprints. In order to quantify the level of the alginate we also report the construction and interpretation of multivariate partial least squares regression models whi...Continue Reading

References

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Aug 16, 2000·Advanced Drug Delivery Reviews·H UludagP A Tresco
Jul 18, 2006·Nucleic Acids Research·Duncan HullTom Oinn
Oct 11, 2007·Pharmacogenomics·David I EllisRoyston Goodacre
Nov 3, 2010·Analytical and Bioanalytical Chemistry·Catherine L WinderRoyston Goodacre

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Citations

Sep 27, 2015·Analytical Biochemistry·Hewen ZhengYan-Yeung Luk
Jun 23, 2015·PloS One·Ashley R BrownJonathan R Lloyd
Jul 14, 2018·PloS One·Haitham AlRabiahRoyston Goodacre
Mar 10, 2015·Applied and Environmental Microbiology·Howbeer MuhamadaliRoyston Goodacre
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samuel CheesemanVi Khanh Truong

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