Rapid regeneration and ploidy stability of 'cv IR36' indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

Botanical Studies
Subramanian Radhesh KrishnanManikandan Ramesh

Abstract

Cereal crops are the major targets for transformation mediated crop improvement and IR36 is an early maturing, high yielding, insect and disease resistant rice variety however, it is abiotic stress sensitive. Hence, development of an efficient and reproducible micropropagation system via somatic embryogenesis and Agrobacterium tumefaciens mediated transformation is prerequisite to develop abiotic stress tolerant IR36. Further, Genetic stability of analysis of plantlets through RAPD and ISSR and Ploidy level through Flow cytometry (FCM) measurement of 2C DNA content is necessary for future application of transformed IR36. In this study, Mature seeds inoculated on (Murashige and Skoog) MS medium with 11.31 μM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.3 μM Kinetin (Kn) had highest callus induction frequency (98%). The highest regeneration frequency (80%) was observed in MS + 13.28 μM Benzyladenine (BA) with 8.06 μM α-naphthalene acetic acid (NAA). Randomly Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Flow Cytometry (FCM) analysis showed no significant variation in the 2C DNA (0.81 pg/2C) content and Ploidy level between wild type IR36 and in vitro maintained rice lines. Of the various OD bacterial...Continue Reading

References

Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·A BarakatG Bernardi
Jan 24, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J DolezelJ Greilhuber
Nov 1, 1950·Proceedings of the National Academy of Sciences of the United States of America·H SWIFT
Oct 12, 2005·Plant Molecular Biology·Gurdev S Khush
Dec 19, 2006·Plant Biotechnology Journal·Tadeusz WroblewskiRichard Michelmore
Sep 15, 2007·Nature Protocols·Jaroslav DolezelJan Suda
Jun 11, 2009·Biotechnology Advances·Abdul Qayyum RaoS Riazuddin
Jan 1, 1995·Plant Cell Reports·G S Oinam, S L Kothari
Apr 1, 1986·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T Abe, Y Futsuhara
Apr 1, 2009·Plant Science : an International Journal of Experimental Plant Biology·Kenjirou Ozawa

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Methods Mentioned

BETA
flow cytometry
transgenic
PCR
phosphotransferase
Fluorescence

Software Mentioned

FACSDiva

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