Rapid relaxation of single frog skeletal muscle fibres following laser flash photolysis of the caged calcium chelator, diazo-2

FEBS Letters
I P Mulligan, C C Ashley

Abstract

Diazo-2 is a calcium chelator based on BAPTA [(1989) J. Biol. Chem., in press], whose electron withdrawing diazoacetyl group may be rapidly (2000 s-1) converted photochemically to an electron donating carboxymethyl group by exposure to near ultraviolet light, producing an increase in its calcium affinity (Kd changes from 2.2 microM to 0.073 microM) without steric modification of the metal binding site. Photolysis of a 2 mM solution of this compound with a brief flash of light from a frequency-doubled ruby laser (347 nm) caused single skinned muscle fibres from the semitendinosus muscle of the frog Rana temporaria to relax with a mean half-time of 60.4 +/- 5 ms (range 30-100 ms, n = 15) at 12 degrees C, which is faster than the relaxation observed in intact muscles (half-time 133 ms at 14 degrees C [(1986) J. Mol. Biol. 188, 325-342]) and similar to the rate of the fast phase of tension decay in intact single fibres (20 s-1 at 10 degrees C [(1982) J. Physiol. 329, 1-20]).

References

Apr 1, 1978·The Journal of Physiology·J R BlinksS R Taylor
Sep 1, 1986·Biophysical Journal·M A Ferenczi
Jul 1, 1984·The Journal of Physiology·M A FerencziD R Trentham
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Citations

Feb 1, 1991·Quarterly Reviews of Biophysics·C C AshleyT J Lea
Nov 2, 2016·Molecular Biology of the Cell·Ruizhen LiX Johné Liu
Jun 5, 1998·The American Journal of Physiology·P A WahrJ A Rall
Oct 20, 2001·Journal of Applied Physiology·G C SieckY S Prakash
Oct 1, 1990·Journal of Clinical Laser Medicine & Surgery·F FrankF Wondrazek
Dec 1, 1992·Journal of Muscle Research and Cell Motility·J Lännergren, A Arner
Sep 2, 1998·The American Journal of Physiology·S J SimnettC C Ashley
Dec 31, 1991·Biochemical and Biophysical Research Communications·R E PalmerC C Ashley

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