Rapid Remodeling of the Host Epithelial Cell Proteome by the Listeriolysin O (LLO) Pore-forming Toxin.

Molecular & Cellular Proteomics : MCP
Julien Karim MaletDavid Ribet

Abstract

Bacterial pathogens use various strategies to interfere with host cell functions. Among these strategies, bacteria modulate host gene transcription, thereby modifying the set of proteins synthetized by the infected cell. Bacteria can also target pre-existing host proteins and modulate their post-translational modifications or trigger their degradation. Analysis of protein levels variations in host cells during infection allows to integrate both transcriptional and post-transcriptional regulations induced by pathogens. Here, we focused on host proteome alterations induced by the toxin Listeriolysin O (LLO), secreted by the bacterial pathogen Listeria monocytogenes. We showed that a short-term treatment with LLO remodels the host cell proteome by specifically decreasing the abundance of 149 proteins. The same decrease in host protein levels was observed in different epithelial cell lines but not in macrophages. We show in particular that this proteome remodeling affects several ubiquitin and ubiquitin-like ligases and that LLO leads to major changes in the host ubiquitylome. Strikingly, this toxin-induced proteome remodeling involves only post-transcriptional regulations, as no modification in the transcription levels of the corr...Continue Reading

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Citations

Nov 18, 2018·Pathogens and Disease·Andreas KühbacherJavier Pizarro-Cerdá
Nov 9, 2019·Clinical & Translational Immunology·Rachel J LundieKirsten A Fairfax
Mar 19, 2020·Cellular Microbiology·Francis Impens, Olivier Dussurget
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Datasets Mentioned

BETA
PXD009339

Methods Mentioned

BETA
pull-down
scraping
in vitro transcription
electrophoresis
neddylation
ubiquitination
deamidation

Software Mentioned

Andromeda
GeneChip Operating Software ( GCOS
Database for Annotation , Visualization and Integrated Discove...
SPlus ArrayAnalyser
MS Viewer
MaxQuant
Perseus

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