Rapid sampling microdialysis as a novel tool for parenchyma assessment during static cold storage and hypothermic machine perfusion in a translational ex vivo porcine kidney model

The Journal of Surgical Research
Karim HamaouiVassilios Papalois

Abstract

Viability assessment during preservation is imperative to avoid unnecessary discard of marginal organs maximizing graft outcomes in kidney transplantation. To address this need, we have developed a novel system based on a rapid sampling microdialysis (rsMD) analyzer allowing continuous tissue monitoring and measurement of metabolic markers of cell damage. Our aim was to develop a tool that allows for accurate assessment of tissue metabolism and organ viability in the preservation period. Twenty-two porcine kidneys subjected to 15 min of warm ischemia underwent either 24 h of static cold storage (SCS) or 10 h of hypothermic machine perfusion (HMP). After preservation, tissue temperature was allowed to passively increase to ambient temperature as an ischemic challenge. Cortical and medullary metabolism was monitored throughout with online measurements of lactate concentrations made every 60 s. On commencement of monitoring, lactate concentrations were successfully detected within 15 mins. During the initial 1.5 h, lactate concentrations were similar during SCS (65 μM) and HMP (124 μM, P > 0.05) but lower after 10 h of SCS (SCS: 68 μM versus HMP: 230 μM, P < 0.001). Warming data suggest a resilience of HMP kidneys to subsequent te...Continue Reading

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Citations

Oct 16, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Angela Sánchez-GuerreroJuan Sahuquillo
Mar 8, 2017·Current Opinion in Organ Transplantation·Thomas MinorAndreas Paul
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Jan 1, 2017·Convergent Science Physical Oncology·Paula CunneaChristina Fotopoulou
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Jan 18, 2018·The Analyst·S A N GowersM G Boutelle
Jan 28, 2021·International Journal of Molecular Sciences·Maria Irene BelliniVito D'Andrea
Oct 28, 2019·Analytical Chemistry·Isabelle C SamperMartyn G Boutelle

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