Rapid screening and identification of cytochalasins by electrospray tandem mass spectrometry

Journal of Mass Spectrometry : JMS
Jeevan K PrasainHiroyuki Osada

Abstract

The cytochalasin class of fungal metabolites was analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS) with the aim of developing a methodology for their rapid identification in microbial extracts. ESI-MS analyses of reference cytochalasins were performed and several product ions were produced in MS/MS experiments on parent ions that are structurally characteristic. A precursor ion search was performed to detect cytochalasins in an ethyl acetate extract of fungal strain RK97-F21. Three cytochalasins were detected and one of the components was identified as epoxycytochalasin H by comparing the tandem mass spectra of the product ions with those of reference compounds. This finding was further validated by LC/MS and LC/MS/MS experiments.

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Citations

Nov 26, 2010·International Journal of Food Sciences and Nutrition·C R Rekha, G Vijayalakshmi
Aug 14, 2012·Rapid Communications in Mass Spectrometry : RCM·Guo-Bo XuZhi-Jun Wu
Jul 12, 2002·Journal of Mass Spectrometry : JMS
Apr 11, 2018·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Luciana M EliasSimone P Lira
Mar 15, 2018·Scientific Reports·Shandiz TehraniJohn C Morrison
Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yongli ZhangMingquan Guo

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