Rapid screening method for intact glucosinolates in Chinese medicinal herbs by using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry in negative ion mode

Rapid Communications in Mass Spectrometry : RCM
Kim-Chung LeeZongwei Cai

Abstract

An optimized method using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) in negative ion mode has been developed for screening different structural classes of intact glucosinolates in six Chinese medicinal herbs. The glucosinolates were extracted with hot methanol/water (70:30 v/v) and separation of the individual glucosinolates was achieved using a reversed-phase C18 column with an aqueous ammonium acetate/methanol gradient. Identification of the intact glucosinolates was based on the detection of compounds with a constant neutral loss of 242 Da corresponding to the combined loss of anhydroglucose (162 Da) and sulfur trioxide (80 Da) in collision-induced dissociation. The structures of the identified glucosinolates were confirmed with the use of group-specific product ions at m/z 195, 241, 259, 275 in their corresponding MS/MS product ion spectra. Differentiation of intact glucosinolates was achieved through their respective retention times and molecular masses as well as the characteristic product ions. The limits of detection were at the low nanogram level per injection, based on constant neutral loss scans. Significant variation in the compositions of intact glucosinolate...Continue Reading

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangG H Posner
May 8, 1987·Journal of Chromatography·F A MellonJ A Pratt
Jan 4, 2001·Drug Metabolism Reviews·S S Hecht
Dec 3, 2003·Analytical and Bioanalytical Chemistry·Zongwei CaiAlbert Lee

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Citations

May 12, 2009·Analytical and Bioanalytical Chemistry·Silvia MillánRamón J Barrio
Nov 26, 2009·The Plant Journal : for Cell and Molecular Biology·Liping GuRobert L Last
Feb 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juan RománAndrea Mahn
Jan 21, 2021·Annual Review of Food Science and Technology·Xianli WuPamela R Pehrsson
Mar 27, 2021·Journal of Chromatography. a·Albatul Y AlmushaytiMichael J Scotter

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