Rapid screening of destruxins by liquid chromatography/mass spectrometry

Journal of Mass Spectrometry : JMS
A JegorovP Sedmera

Abstract

A liquid chromatographic/mass spectrometric (LC/MS) method utilizing an atmospheric pressure chemical ionization (APCI) interface and in-source collisionally induced dissociation (CID) was developed for the rapid screening of the cyclic hexadepsipeptides destruxins, produced by the fungi Metarhizium anisopliae and Trichothecium roseum. The APCI mass spectra are fully consistent with the high-energy CID data but the sequence ions are abundant over the whole mass range. In addition to 22 known destruxins, two new representatives of this family were detected by LC/MS analysis: destruxin Ed1 and roseotoxin A were isolated and their structures were inferred from MS/MS and NMR data.

Citations

Jul 2, 2011·Toxicon : Official Journal of the International Society on Toxinology·Xiang MengShunxiang Ren
Nov 15, 2011·Biotechnology Advances·Bing-Lan Liu, Yew-Min Tzeng
Aug 15, 2002·Archives of Biochemistry and Biophysics·Maria HinajeBhupinder Khambay
Aug 31, 2002·Rapid Communications in Mass Spectrometry : RCM·Simone CristoniFederico Guidugli
Mar 11, 2017·Analytical and Bioanalytical Chemistry·Natalia Arroyo-ManzanaresSarah De Saeger
Mar 13, 2003·Folia Microbiologica·T Rezanka, J Spizek
Jul 6, 2010·Natural Product Reports·István MolnárStuart B Krasnoff

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