Rapid screening of highly efficient vaccine candidates by immunoproteomics

Proteomics
Zijun ChenXuanxian Peng

Abstract

Diseases caused by microorganisms can be controlled by vaccines, which require neutralizing antigens. Therefore, it is very important to identify highly efficient immunogens for immune prevention. By combining immunoproteomics and bacterial challenge after immunization, we developed a rapid method for screening protected antigens of pathogenic bacteria in aquaculture. Our approach may be divided into three consecutive steps. First, dominant immunogens of outer membrane proteins are screened by immunoproteomics. Second, proteins with the ability to induce production of neutralizing antibodies are identified from the immunogens by virulent bacterium challenge following vaccination. Third, vaccine candidates are determined by evaluation of neutralizing abilities. Information on the candidates has been obtained for further gene cloning by mass spectrometry. Our results indicate that highly efficient protected antigens were identified from the outer membrane proteome of Aeromonas hydrophila, in which an immunogen showed 71.4% protective ability with multivalent functions to A. hydrophila and Aeromonas sobria. In summary, we have developed a high-throughout, accurate, rapid and highly efficient method which will play an active role i...Continue Reading

Citations

Apr 12, 2005·Life Sciences·Yueguang FanXuanxian Peng
May 21, 2008·Microbial Pathogenesis·Saravanane PoobalaneAlexandra Adams
Feb 10, 2007·Vaccine·Sarah V HardingRichard W Titball
Feb 1, 2008·Proteomics. Clinical Applications·Harold TjalsmaDorine W Swinkels
Apr 28, 2012·Proteomics·Bernd GesslbauerAndreas J Kungl
Nov 30, 2004·Research in Microbiology·Changxin XuXuanxian Peng
Mar 28, 2006·Trends in Biotechnology·Alexandra Adams, Kim D Thompson
May 26, 2005·International Immunopharmacology·Changxin XuXuanxian Peng
Apr 14, 2012·Journal of Proteomics·Pedro M RodriguesFlemming Jessen
Jul 12, 2008·FEMS Microbiology Letters·Ying MaoChengping Lu
Aug 12, 2010·Journal of Applied Microbiology·H-Y PangS-H Cai
Jun 14, 2020·Pathogens·Saúl Rojas-HernándezMaría Maricela Carrasco-Yépez
Jul 18, 2008·Journal of Proteome Research·Hongran GengYongqiang Jiang

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