Rapid screening of marine bacterial symbionts using MALDI-TOF MS

Archives of Microbiology
Livia M R VidalCristiane C Thompson

Abstract

Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria. MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups' identification.

References

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Citations

May 14, 2021·World Journal of Microbiology & Biotechnology·Grazielly Maria Didier de VasconcelosCristiano José de Andrade

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Software Mentioned

Mass Up
Biotyper
BLAST
Chromas Pro
FlexAnalysis
Biotyping
XLSTAT

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