Rapid Screening of Urinary Tract Infection and Discrimination of Gram-Positive and Gram-Negative Bacteria by Automated Flow Cytometric Analysis Using Sysmex UF-5000

Journal of Clinical Microbiology
Seon Young KimGye Cheol Kwon

Abstract

Rapid screening of urinary tract infection is important to determine antibiotic treatment and reduce unnecessary urine culture. We evaluated the performance of the new flow cytometry-based UF-5000 automated urine analyzer (Sysmex, Kobe, Japan). A total of 1,430 urine samples from 1,226 patients were analyzed and compared to urine cultures to which a Previ Isola (bioMérieux, Marcy l'Etoile, France) system was applied. In total, 878 of 1,430 urine cultures (61.4%) produced ≥103 CFU/ml bacterial growth (309 with Gram-negative [GN] bacteria, 517 with Gram-positive [GP] bacteria, and 52 mixed cultures), with 336 samples (23.5%) presenting ≥105 CFU/ml bacterial growth. The ≥105 CFU/ml bacterial growth was detected by a ≥71 bacteria/μl UF-5000 bacterial count with 95% sensitivity and 84% specificity. Using a cutoff of <15 bacteria/μl to determine whether or not to culture, 50.9% of samples were below the cutoff, 94.8 and 99.5% of which presented <104 and <105 CFU/ml of bacterial growth, respectively. The bacterial discrimination performance of the UF-5000 for GN bacteria was superior to that for GP bacteria, and in ≥105 CFU/ml monobacterial samples, the sensitivity and specificity for reporting GN bacteria were 91.7 and 90.0%, respect...Continue Reading

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Citations

Dec 6, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Eline S AndersenIvan Brandslund
Jun 7, 2019·The Journal of Antimicrobial Chemotherapy·Justin R Lenhard, Zackery P Bulman
Feb 9, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Eline Sandvig Andersen, Ivan Brandslund
Feb 8, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Timo KouriAnu Pätäri-Sampo

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