Rapid screening test to estimate temperature optima for microalgae growth using photosynthesis activity measurements

Folia Microbiologica
Karolína RanglováJiří Masojídek

Abstract

We have worked out a rapid 1-day test based on photosynthesis measurements to estimate suitable growth temperature of microalgae cultures. To verify the proposed procedure, several microalgae-Chlorella, Nostoc, Synechocystis, Scenedesmus, and Cylindrospermum-were cultured under controlled laboratory conditions (irradiance, temperature, mixing, CO2, and nutrient supply) to find the optima of photosynthetic activity using the range between 15 and 35 °C. These activities were recorded at each temperature step after 2 h of acclimation which should be sufficient as oxygen production and the PQ cycle are regulated by fast processes. Photosynthetic activity was measured using three techniques-oxygen production/respiration, saturating pulse analysis of fluorescence quenching, and fast fluorescence induction kinetics-to estimate the temperature optima which should correspond to high growth rate. We measured all variables that might have been directly related to growth-photosynthetic oxygen evolution, maximum photochemical yield of PSII, Fv/Fm, relative electron transport rate rETRmax, and the transients Vj and Vi determined by fast fluorescence induction curves. When the temperature optima for photosynthetic activity were verified in gr...Continue Reading

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Mar 10, 2016·Photosynthesis Research·Vinzenz Bayro-Kaiser, Nathan Nelson

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Citations

Aug 7, 2019·Folia Microbiologica·Ondřej Prášil
Apr 24, 2021·Life Sciences in Space Research·Bo WangGaohong Wang
Jan 15, 2020·Journal of Agricultural and Food Chemistry·Mykola MylenkoPavel Hrouzek

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