Rapid selection for β-tubulin alleles in codon 200 conferring benzimidazole resistance in an Ostertagia ostertagi isolate on pasture

Veterinary Parasitology
Friederike Knapp-LawitzkeJanina Demeler

Abstract

Resistance to benzimidazoles (BZs) is widespread in sheep nematodes and increasing in those of cattle. Several reasons including the predominant use of pour-on anthelmintics and lack of scales in field conditions lead to under-dosing of cattle and therefore to increased selection pressure. In an field experiment the frequency of BZ-resistance associated allele (TAC) in codon 200 in the β-tubulin isotype 1 gene of Ostertagia ostertagi was monitored over one grazing season (approximately 30 weeks). Group 1, consisting of four calves, was experimentally infected with a pure O. ostertagi population displaying ∼50% of the TAC allele. The subsequently following groups of calves (four groups of two calves each) acquired natural infections by grazing contaminated pastures. Each group was treated with increasing percentages of sub-therapeutic dosages of albendazole (35-65%). Larvae obtained from faecal cultures pre and post treatment were subjected to species/genus-specific PCR as well as pyrosequencing to determine allele frequencies. PCR revealed the presence of Ostertagia, Trichostrongylus, Haemonchus and Cooperia in pre-treatment samples and predominantly Ostertagia as well as some Trichostrongylus in post treatment samples. Faecal ...Continue Reading

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Apr 29, 2014·Experimental Parasitology·Friederike Knapp-LawitzkeJanina Demeler
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Citations

Jun 25, 2016·Acta Tropica·Luis Fernando Viana FurtadoÉlida Mara Leite Rabelo
May 19, 2017·Parasitology·B TarbiatJ Höglund
Nov 28, 2017·BMC Veterinary Research·Lauren Hubert Jaeger, Filipe Anibal Carvalho-Costa
Feb 9, 2019·The American Journal of Tropical Medicine and Hygiene·Ambrose R OrrMichael Cappello
Dec 11, 2019·Parasites & Vectors·Pablo D Jimenez CastroRay M Kaplan
Aug 4, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Georg von Samson-HimmelstjernaPeter Deplazes
Oct 26, 2020·Preventive Veterinary Medicine·Flávia C FáveroFernando de A Borges

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