Rapid semiautomated screening and processing of urine specimens.

Journal of Clinical Microbiology
R D JenkinsJ M Matsen

Abstract

A rapid urine culture procedure was evaluated in which positive urines were detected by using light-scatter photometry (Autobac). Specimens were analyzed at 3, 5, and 6 h. Specimens detected as positive at 3 h were then further evaluated by a direct 3-h susceptibility procedure (Autobac) and by a 4-h identification procedure (Micro-ID). Of 949 specimens, 175 had >10(5) colony-forming units per ml by colony count. Of these latter specimens, 75.4% had been detected by 3 h, and 95.4% were detected by 6 h. Of specimens positive by Autobac at 3 h, 96% (95.7%) had >10(5) colony-forming units per ml. If pure by Gram stain, those positive specimens were inoculated to direct susceptibility and identification systems. When direct Autobac susceptibilities were compared with the standard Autobac method done from the plate the following day, discrepancy rates were 1.3% very major, 2.1% major, and 7.4% total. The direct identifications were 94% (94.2%) correct when using the Micro-ID manual and a collection of octal patterns unique to this system, in which urine/broth culture inoculum was employed instead of the usual organism colony suspension. Those urine specimens negative after screening at 3 h were evaluated at 5 and 6 h, and an additio...Continue Reading

References

Apr 11, 1975·Antimicrobial Agents and Chemotherapy·C ThornsberryJ A Washington

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Citations

Jan 1, 1982·Medical Microbiology and Immunology·P C Appelbaum, C C Olmstead
Aug 1, 1982·European Journal of Clinical Microbiology·P W McWalter, C A Sharp
Mar 2, 2017·Nature Reviews. Urology·Michael DavenportJoseph C Liao
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Sep 1, 1995·Journal of Clinical Microbiology·J R JohnsonW E Stamm
Jul 28, 1983·The American Journal of Medicine·M T Pezzlo

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